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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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杨树magpie和jackdaw基因克隆、表达及蛋白互作研究
DOI: 10.3969/j.issn.1000-2006.2014.03.006, PP. 29-34
Keywords: 杨树不定根,基因表达模式,蛋白亚细胞定位,双分子荧光互补分析
Abstract:
利用race技术从杨树不定根中克隆获得pemgp和pejdk基因全长cdna序列,分别包含1434bp和1518bp长度的开放阅读框。两者都含2个内含子,其氨基酸序列均有4个保守结构域:2个c2h2型锌指结构域zf1(cx2-4cx3fx4lx2hx3-5h)和zf2(cx4cxnhxnx4h),2个c2hc型锌指结构域zf3(cx2cxnhx3c)和zf4(cxcxnhx3c)。在根茎叶中均检测到pemgp和pejkd基因的表达,但它们在杨树不定根发育过程中具有动态的表达模式。杨树原生质体瞬时表达揭示,pemgp和pejdk蛋白都定位于细胞核,两者在细胞核发生相互作用。
| [1] | cruz-ramíreza,díaz-triviños,bliloui,etal.abistablecircuitinvolvingscarecrow-retinoblastomaintegratescuestoinformasymmetricstemcelldivision[j].cell,2012,150(5):1002-1015.
|
| [2] | azpeitiae,weinsteinn,bentezm,etal.findingmissinginteractionsofthearabidopsisthalianarootstemcellnichegeneregulatorynetwork[j].frontiersinplantscience,2013,110(4):1-20.
|
| [3] | welchd,hassanh,bliloui,etal.arabidopsisjackdawandmagpiezincfingerproteinsdelimitasymmetriccelldivisionandstabilizetissueboundariesbyrestrictingshort-rootaction[j].genes&development,2007,21:2196-2204.
|
| [4] | 宋冰,洪洋,王丕武,等.植物c2h2型锌指蛋白的研究进展[j].基因组学与应用生物学,2010,29(5):1133-1141.songb,hongy,wangpw,etal.advancesonplantc2h2-typezincfingerprotein[j].genomicsandappliedbiology,2010,29(5):1133-1141.
|
| [5] | colasantij,tremblayr,wongay,etal.themaizeindeterminate1floweringtimeregulatordefinesahighlyconservedzincfingerproteinfamilyinhigherplants[j].bmcgenomics,2006,158(7):1-17.
|
| [6] | hassanh,scheresb,bliloui.jackdawcontrolsepidermalpatterninginthearabidopsisrootmeristemthroughanon-cell-autonomousmechanism[j].development,2010,137(9):1523-1529.
|
| [7] | tamurak,petersond,petersonn,etal.mega5:molecularevolutionarygeneticsanalysisusingmaximumlikelihood,evolutionarydistance,andmaximumparsimonymethods[j].molecularbiologyandevolution,2011,28(10):2731-2739.
|
| [8] | xum,zhangb,sux,etal.referencegeneselectionforquantitativereal-timepolymerasechainreactioninpopulus[j].analyticalbiochemistry,2011,408(2):337-339.
|
| [9] | tanb,xum,cheny,etal.transientexpressionforfunctionalgeneanalysisusingpopulusprotoplasts.[j].plantcell,tissueandorganculture(pctoc),2013,114(1):11-18.
|
| [10] | yangm,mayws,itot.jazrequiresthedouble-strandedrna-bindingzincfingermotifsfornuclearlocalization[j].journalofbiologicalchemistry,1999,274(39):27399-27406.
|
| [11] | 李少锋,苏晓华,张冰玉.林木基因克隆研究进展[j].植物学报,2011,46(1):79-107.lisf,suxh,zhangby.researchprogressingenecloninginforesttrees[j].chinesebulletinofbotany,2011,46(1):79-107.
|
| [12] | huangj,yangx,wangmm,etal.anovelricec2h2-typezincfingerproteinlackingdln-box/ear-motifplaysaroleinsalttolerance[j].biochimicaetbiophysicaacta(bba)-genestructureandexpression,2007,1769(4):220-227.
|
| [13] | sakamotoh,maruyamak,sakumay,etal.arabidopsiscys2/his2-typezinc-fingerproteinsfunctionastranscriptionrepressorsunderdrought,cold,andhigh-salinitystressconditions[j].plantphysiology,2004,136(1):2734-2746.
|
| [14] | 郭书巧.水稻锌指蛋白基因rzf5和rzf71的克隆与功能分析[d].南京:南京农业大学,2006.guosq.cloningandfunctionalanalysisoftwozincfingerproteingenes,rzf5andrzf71fromrice(oryzasatival.)[d].nanjing:nanjingagriculturaluniversity,2006.
|
| [15] | bowmanjl,sakaih,jackt,etal.superman,aregulatoroffloralhomeoticgenesinarabidopsis[j].development,1992,114(3):599-615.
|
| [16] | chrispeelshe,oettingerh,janviern,etal.atzfp1,encodingarabidopsisthalianac2h2zinc-fingerprotein1,isexpresseddownstreamofphotomorphogenicactivation[j].plantmolecularbiology,2000,42(2):279-290.
|
| [17] | 胥猛.杨树不定根发育的基因表达调控研究[d].南京:南京林业大学,2008.xum.researchongeneexpressionandregulationofpopulusadventitionsrootdevelopment[d].nanjing:nanjingforestryuniversity,2008.
|
| [18] | levesquemp,vernouxt,buschw,etal.whole-genomeanalysisoftheshort-rootdevelopmentalpathwayinarabidopsis[j].plosbiology,2006,4(5):e143.
|
| [19] | 高潜,刘玉瑛,费一楠,等.拟南芥根的辐射形态相关基因short-root研究进展[j].植物学通报,2008,25(3):363-372.gaoq,liuyy,feiyn,etal.researchadvancesintotherootradialpatterninggeneshort-root[j].chinesebulletinofbotany,2008,25(3):363-372.
|
| [20] | 胥猛,谢雯凡,潘惠新,等.杨树argonaute基因的克隆及序列分析[j].林业科学,2011,47(3):46-51.xum,xiewf,panhx,etal.cloningandcharacterizationofargonautegenesinpopulus[j].scientiasilvaesinicae,2011,47(3):46-51.
|
| [21] | huangj,wangj,zhangh.ricezfp15geneencodingforanovelc2h2-typezincfingerproteinlackingdlnbox,isregulatedbyspikedevelopmentbutnotbyabioticstresses[j].molecularbiologyreports,2005,32(3):177-183.
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