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OALib Journal期刊
ISSN: 2333-9721
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杉木成年优树组培生根研究
DOI: 10.3969/j.issn.1000-2006.2013.06.034, PP. 169-172
周小红, 张元莉, 李美平, 赵芳芳, 陈金慧, 郑仁华, 肖晖, 黄金华, 张志才, 李勇, 郑雪燕, 施季森*
Keywords: 杉木,器官发生,生根,基因型
Abstract:
以3个成年杉木优良无性系茎段为外植体,研究基因型、生长调节剂、活性炭、基本培养基对杉木生根效率的影响。结果表明:基因型和生长素naa是影响杉木生根效率的主要因素。1#基因型的杉木生根能力强,在1/4dcr培养基(添加iba1.0mg/l或naa0.5～1.0mg/l)上生根率可达90%以上;2#基因型的杉木生根能力较弱,添加1.5mg/liba或1.0mg/lnaa的1/2dcr培养基更适合其生根;对于3#基因型的杉木,3/4dcr培养基最适合其生根,添加iba1.5mg/l或naa1.0mg/l时生根率最高。对3个基因型的生根试验研究表明,活性炭对杉木生根不能起到促进作用。
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