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OALib Journal期刊
ISSN: 2333-9721
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青钱柳茎段腋芽的离体培养技术
DOI: 10.3969/j.issn.1000-2006.2013.06.002, PP. 6-10
Keywords: 青钱柳,植物生长调节剂,腋芽,增殖系数,生根诱导
Abstract:
为建立高效的青钱柳快速繁殖体系,以青钱柳茎段腋芽为外植体,wpm培养基为基本培养基,研究了不同植物生长调节剂对青钱柳腋芽萌发、丛生芽增殖及生根诱导的影响。结果表明:在腋芽萌发过程中,9个组合处理对腋芽萌发均有促进作用,其中5mg/l6-ba+1.5mg/lkt+0.1mg/lnaa为最佳组合,腋芽萌芽率86.67%;腐胺具有促进腋芽萌动,缩短萌芽周期的作用;丛生芽增殖培养中,以0.3mg/ltdz+0.3mg/l6-ba增殖效果最佳,增殖系数为6.78;生根诱导中,10mg/lggr-6+1.0mg/liba组合处理效果最好,生根率为25%,生长状态优于其他处理。
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