|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
湿加松胚性愈伤组织的程序降温技术研究
DOI: 10.3969/j.issn.1000-2006.2013.06.001, PP. 1-5
Keywords: 湿加松,超低温冷冻保存,胚性愈伤组织
Abstract:
对湿加松胚性愈伤组织进行了超低温冷冻保存的研究。结果表明:继代培养9～12d的湿加松胚性愈伤组织经0.5mol/l山梨醇预培养4d,在0.6mol/l山梨醇+10%dmso的冷冻保护液下0℃预处理20min,然后以-1℃/min的降温速率降至-40℃,停留10min后再以-5℃/min的降温速率降至-90℃,投放入液氮中保存;复苏时于37℃水浴2min,1mol/l山梨醇的液体培养基清洗3次,以滤纸作支持物转到固体继代培养基再培养。在此程序处理下1个月内可获得生长状态良好的再生愈伤组织。
| [1] | 方利娟.杂交松组织培养技术研究[d].广西:广西大学,2006.fanglj.studyontechnologyaboutthetissuecultureofpinuselliottii×p.caribaea[d].guangxi:guangxiuniversity,2006.
|
| [2] | 施翔.杂交松组织培养研究[d].南京:南京林业大学,2005.shix.studyontissuecultureofpinuselliottii×p.caribaea[d].nanjing:nanjingforestryuniversity,2005.
|
| [3] | 项东云,王以红,吴幼媚,等.杂交松组培技术研究初探[j].广西林业科学,2009,38(3):134-136.xiangdy,wangyh,wuym,etal.preliminarystudyontissuecultureofpinuselliottii×p.caribaea[j].guangxiforestryscience,2009,38(3):134-136.
|
| [4] | 施季森,于敏,边黎明,等.湿地松和加勒比松杂种体细胞胚胎发生和植株再生方法.cn200710132707.8[p].北京:知识产权出版社,2008.shijs,yum,bianlm,etal.methodforsomaticembryogenesisandplantregenerationofhybridbetweenpinuselliottiandpinuscaribaea.cn200710132707.8[p].beijing:intellectualpropertypublishinghouse,2008.
|
| [5] | 李峰卿.湿加杂种松体细胞胚胎发生同步化控制和植株再生[d].南京:南京林业大学,2009.lifq.synchronizationcontrollingsystemfortheembryogenesisproliferationandplantletsregenerationofthenewhybridbetweenpinuselliottiiandpinuscaribaea[d].nanjing:nanjingforestryuniversity,2009.
|
| [6] | 叶芳,夏胜,梅兴国.红豆杉愈伤组织超低温保存有关因素的研究[j].武汉植物学研究,2001,19(4):327-331.yef,xias,meixg.studiesonfactorsincryopreservationoftaxuscallus[j].journalofwuhanbotanicalresearch,2001,19(4):327-331.
|
| [7] | nievesv,conchis,lorenaj,etal.cryopreservationofchestnutbyvitrificationofinvitro-grownshoottips[j].invitrocellular&developmentalbiology-plant,2005,41(1):63-68.
|
| [8] | 史峰厚,喻方圆,沈永宝,等.超低温贮存对油松种子的影响[j].南京林业大学学报:自然科学版,2005,29(6):119-122.shifh,yufy,shenyb,etal.cryopreservationofchinesepineseeds[j].journalofnanjingforestryuniversity:naturalsciencesedition,2005,29(6):119-122.
|
| [9] | 任淑娟,王航,刘浩,等.七叶树种子离体胚的超低温冷冻保存[j].南京林业大学学报:自然科学版,2010,34(2):19-23.rensj,wangh,liuh,etal.cryopreservationofexcisedembryosofaesculuschinensisseeds[j].journalofnanjingforestryuniversity:naturalsciencesedition,2010,34(2):19-23.
|
| [10] | 苗琦,谷运红,王卫东,等.植物组织培养物的超低温保存[j].植物生理学通讯,2005,41(3):350-354.miaoq,guhy,wangwd,etal.thecryopreservationofplanttissueculturematerials[j].plantphysiologyjournal,2005,41(3):350-354.
|
| [11] | 关静,龚承元,崔占峰.玻璃化冷冻保存细胞组织研究进展[j].国外医学:生物医学工程分册,2004,27(4):252-256.guanj,gongcy,cuizf.recentadvancesincryopreservationofcellsandtissuesbyvitrification[j].internationaljournalofbiomedicalengineering,2004,27(4):252-256.
|
| [12] | 翟晓巧,程斐,朱延林.二乔刺槐愈伤组织超低温保存及适宜降温方法[j].林业科学,2009,45(10):49-54.zhaoxq,chengf,zhuyl.methodsofloweringtemperatureforcryopreservationofcalliofrobiniabella-rosea[j].scientiasilvaesinicae,2009,45(10):49-54.
|
| [13] | 刘剑锋,阎秀峰,程云清,等.高山红景天愈伤组织的超低温保存[j].林业科学,2007,43(6):57-60.liujf,yanxf,chengyq,etal.cryopreservationofcallusofrhodiolasachalinensis[j].scientiasilvaesinicae,2007,43(6):57-60.
|
| [14] | barbaramreed.plantcryopreservation:apracticalguide[m].newyork:springer,2007.
|
| [15] | malabadirb,natarajak.cryopreservationandplantregenerationviasomaticembryogenesisusingshootapicaldomesofmaturepinusroxburghiisarg.trees[j].invitrocellular&developmentalbiology-plant,2006,42(2):152-159.
|
| [16] | mathurg,alkuterva,nadgaudars.cryopreservationofembryogeniccultureofpinusroxburghii[j].biologiaplantarum,2003,46(2):205-210.
|
| [17] | salajt,panisb,swennenr,etal.cryopreservationofembryogenictissuesofpinusnigraarn.byaslowfreezingmethod[j].cryoletters,2007,28(2):69-76.
|
| [18] | gales,johna,bensonee.cryopreservationofpiceasitchensis(sitkaspruce)embryogenicsuspensormasses[j].cryoletters,2007,28(4):225-239.
|
| [19] | krlepk,bergantm,dewintergm.cryopreservationofcellsuspensionculturesoftaxusmediaandtaxusfloridana[j].biologiaplantarum,2008,52(2):329-333.
|
| [20] | 陈代喜,莫钊志,李贵玉,等.杂交松育苗对比试验效果分析[j].广西林业科学,2002,31(2):76-77.chendx,mozz,ligy,etal.thecontrolledexperimentonpinuselliottii×pinusoaribaeaseedling-raising[j].guangxiforestryscience,2002,31(2):76-77.
|
| [21] | 庞正轰,陈代喜,莫钊志,等.澳大利亚杂交松产脂量及松脂成分研究[j].林业科技开发,2009,23(6):23-26.pangzh,chendx,mozz,etal.studyontheoutputofresinanditschemicalcompositionfromaustraliapinehybrid[j].chinaforestryscienceandtechnology,2009,23(6):23-26.
|
| [22] | 凌绍明.澳大利亚杂交松在广西引种试验研究[j].广西林业科学,2007,36(4):192-195.lingsm.astudyontheintroductionofaustraliahybridpineinguangxi[j].guangxiforestryscience,2007,36(4):192-195.
|
| [23] | 施季森.迎接21世纪现代林木生物技术育种的挑战[j].南京林业大学学报,2000,24(1):1-6.shijs.thechallengestoforestrybiotechnologyandtreebreedinginthe21stcentury[j].journalofnanjingforestryuniversity,2000,24(1):1-6.
|
| [24] | 邵崇斌.概率论与数理统计[m].北京:中国林业出版社,2005.
|
| [25] | 徐刚标,易文,李美娥,等.银杏愈伤组织超低温保存的研究[j].林业科学,2001,37(3):30-34.xugb,yiw,lime,etal.thestudiesofcryopreservationofcallusformaidenhairtree(gingkobiloball.)[j].scientiasilvaesinicae,2001,37(3):30-34.
|
| [26] | karlssonjo,tonerm.long-termstorageoftissuesbycryopreservation:criticalissues[j].biomaterials,1996,17(3):243-256.
|
| [27] | 李广武,郑从义,唐兵.低温生物学[m].长沙:湖南科学技术出版社,1998.
|
| [28] | 叶芳,夏胜,梅兴国.红豆杉愈伤组织超低温保存有关因素的研究[j].武汉植物学研究,2001,19(4):327-331.yef,xias,meixg.studiesonfactorsincryopreservationoftaxuscallus[j].journalofwuhanbotanicalresearch,2001,19(4):327-331.
|
| [29] | 王高,范现丽,申晓辉,等.红松胚性愈伤组织的超低温保存技术[j].上海交通大学学报:农业科学版,2009,27(3):223-230.wangg,fanxl,shenxh,etal.cryopreservationofkoreanpinesomaticcalli[j].journalofshanghaijiaotonguniversity:agriculturalscience,2009,27(3):223-230.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
