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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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黑曲霉外切葡聚糖酶基因密码子的优化及表达
DOI: 10.3969/j.jssn.1000-2006.2012.04.017, PP. 84-88
Keywords: 外切葡聚糖酶,定点突变,密码子优化,黑曲霉,毕赤酵母
Abstract:
通过ncbi(美国国立生物技术信息中心)对黑曲霉的来源外切葡聚糖酶基因(cbh1)的氨基酸序列进行分析,发现其16个活性部位中有6个位点附近的氨基酸为毕赤酵母(pichiapastoris)表达的稀有密码子。利用反向长距离pcr技术将这些活性位点附近的稀有密码子定点突变为毕赤酵母表达偏爱密码子。将优化后的表达载体ppiczαa-cbh1m转入毕赤酵母km71h菌株中进行诱导表达。经密码子优化后的cbh1m基因较未优化前cbh1基因的表达量提高了17%。
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