OALib Journal期刊
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杨树黑斑病菌?marssoninabrunnea?的?有丝分裂过程和染色体数观察
DOI: 10.3969/j.jssn.1000-2006.2012.01.005, PP. 21-27
Keywords: 杨树黑斑病菌,细胞学,核型,有丝分裂,芽管萌发技术(gtbm)
Abstract:
利用芽管萌发技术(gtbm)对杨树黑斑病菌?marssoninabrunnea?分生孢子萌发过程中的有丝分裂过程进行了观察,?m.brunnea?的有丝分裂过程可以分为4个时期:前期染色质逐渐浓缩变短,中期可清楚观察得到染色体,后期姐妹染色单体发生分离并分别向两极移动,末期则可见子核的形成。另外,利用dapi、dapi/pi和giemsa3种不同的染色方法对染色体进行观察比较,结果表明:giemsa染色法最适用于?m.brunnea?;在?m.brunnea?有丝分裂中期可清楚观察到3条染色体。结合3种染色法,比较分析染色体的绝对值大小和相对值大小,其中相对值大小更加清晰和稳定地描述不同染色体间的变化。?
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