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OALib Journal期刊
ISSN: 2333-9721
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转tabzip基因白桦的获得及耐盐性分析
DOI: 10.3969/j.issn.1000-2006.2013.05.002, PP. 6-12
Keywords: 白桦,tabzip基因,生理指标,耐盐性
Abstract:
植物碱性亮氨酸拉链(bzip)蛋白是一类重要的植物转录因子，该蛋白可与顺式作用元件abre等相互作用，使下游许多抗旱、耐盐等抗性相关基因得以表达，从而提高植物的抗逆性。为了获得耐盐白桦转基因新品系，采用农杆菌介导法,将来自于柽柳的转录因子tabzip基因导入白桦基因组，经qrt-pcr及westernblot检测证明获得了6个转基因株系。在质量分数为0.4%的nacl胁迫条件下，比较了转基因株系的相对高生长、盐害指数及叶绿素荧光参数等指标。结果显示：盐胁迫后tb4株系的相对高生长量最大，达到52.4%，较对照(ck)提高了73.5%；且它的盐害指数仅为0.05，盐伤害最轻，而非转基因对照株系为0.19；nacl胁迫后，转基因株系tb4的叶绿素荧光参数不同于其他株系，它的非光化学淬灭参数(qnp)最大，实际光化学效率(φpsⅱ)及光化学淬灭系数(qp)的降幅最小，而最大光量子效率参数(fv/fm)略高于胁迫前。这些指标的变化均证实外源tabzip的表达能够提高tb4转基因株系的耐盐能力。
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