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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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杨四瘿螨诱导的美洲黑杨抑制消减文库
DOI: 10.3969/j.jssn.1000-2006.2009.02.002
Keywords: 杨四瘿螨,美洲黑杨,抑制性消减杂交技术
Abstract:
采用抑制性消减杂交(suppressionsubtractivehybridization，ssh)技术，以杨四瘿螨诱导48、72h的美洲黑杨无性系为实验方(tester)，以未经诱导的美洲黑杨无性系为驱动方(driver)构建杨四瘿螨诱导差异表达的抑制消减cdna文库，共获得了710个克隆，所建cdna文库的插入片段主要集中在200~1000bp之间。通过序列测定拼接和同源性对比分析，获得了美洲黑杨涉及信号传递、能量代谢、胁迫响应、物质运输等功能的差异基因。
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