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OALib Journal期刊
ISSN: 2333-9721
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铁线莲‘multi？blue’体细胞胚诱导和植株再生
DOI: 10.3969/j.jssn.1000-2006.2010.06.005, PP. 18-22
Keywords: 铁线莲‘multi？blue’,体细胞胚,植株再生
Abstract:
以铁线莲品种‘multi？blue’的茎尖为外植体，1/2ms为基本培养基，进行胚性愈伤组织和体细胞胚的诱导及植株再生条件的选择。结果表明：茎尖外植体在1/2ms+tdz(1.0mg/l)+naa(0.5mg/l)+2%蔗糖+0.7%琼脂的固体培养基中培养时可形成淡黄色块状至颗粒状愈伤组织，经继代培养后可逐渐形成胚性愈伤组织；体细胞胚诱导以1/2ms+tdz(0.2mg/l)+iba(0.1mg/l)+aba(0.3mg/l)+ch(0.5g/l)为佳，诱导率可达96%；而适宜的体细胞胚萌发培养基为1/2ms+6-ba(0.2mg/l)+iba(0.05mg/l)。体胚再生植株经驯化后，移栽成活率可达80%以上。
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