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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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利用环介导恒温扩增法简易诊断松萎蔫病takuyaaikawa,natsumikanzak,taiseikikuch
DOI: 10.3969/j.jssn.1000-2006.2011.04.032, PP. 148-149
Keywords: 松萎蔫病,松材线虫,dna,环介导恒温扩增法
Abstract:
诊断松萎蔫病的传统方法是从木质组织中分离松材线虫，然后用显微镜进行形态学鉴定。基于dna分子检测的方法尽管很灵敏，不需要固定发育阶段的松材线虫，但是仍然需要用baermann漏斗法分离线虫，并且需要昂贵的仪器。笔者研发了利用环介导恒温扩增法检测松材线虫的存在,该方法包括：(1)从木块中提取dna；(2)dna扩增；(3)通过反应溶液的颜色做出诊断。该方法仅使用培养箱且整个过程只需要90min,比以往方法更加简便和快速。
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