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OALib Journal期刊
ISSN: 2333-9721
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纤维素酶液中β-葡萄糖苷酶的分离纯化
DOI: 10.3969/j.jssn.1000-2006.2011.04.023, PP. 111-116
Keywords: 里氏木霉,黑曲霉,纤维素酶,β-葡萄糖苷酶,分离纯化
Abstract:
通过(nh4)2so4分级沉淀、hiprep26/10desalting脱盐柱、source15q阴离子交换柱、source15s阳离子交换柱、hitrap16/60sephacryls？200hr凝胶过滤等技术，分离纯化3种来源里氏木霉、黑曲霉、里氏木霉与黑曲霉混合纤维素酶液中的β-葡萄糖苷酶。结果表明，经sds？page电泳鉴定均为电泳纯，测得黑氏木霉、黑曲霉单独培养β-葡萄糖苷酶相对分子质量分别为68、129ku，而混合菌培养分离得到两种β-葡萄糖苷酶分子质量大小分别为66.2、134ku。与单独培养的β-葡萄糖苷酶相似。3种来源的β-葡萄糖苷酶经多步分离纯化后的纯化倍数分别为37.25、40.21、30.12，酶活回收率分别为20.12%、23.21%、28.56%。
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