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斑翅果蝇幼虫人工饲料的优化配方
DOI: 10.3969/j.issn.1000-2006.2015.04.031
吴 ,
军 ,
骆亚琴 ,
廖太林 ,
曾 ,
虹 ,
师振华 ,
陈集翰 ,
纪 ,
睿 ,
安榆林
Keywords: 斑翅果蝇 , 幼虫 , 人工饲料 , 优化配方Drosophila suzukii , larva , artificial diet , optimization formula
Abstract:
为规模化人工饲养斑翅果蝇,以斑翅果蝇幼虫存活率、幼虫历期、蛹平均质量以及羽化率作为考察指标,采用正交试验设计,对其幼虫人工饲料3种主要成分(玉米颗粒、蔗糖、酵母浸粉)用量进行优化筛选。结果表明:玉米颗粒对斑翅果蝇幼虫存活率有显著影响,蔗糖对蛹平均质量有显著影响,酵母浸粉对幼虫历期有显著影响; 斑翅果蝇幼虫基础人工饲料中的最优质量配比为m(玉米颗粒):m(蔗糖):m(酵母浸粉)=9:16:3。In order to provide the basis for large-scale artificial breeding of Drosophila suzukii Matsumura, the composition of artificial diet for larvae was optimized by orthogonal design with 3 factors and 3 levels. Corn particle, sucrose and yeast extract were selected as the three key factors to determine their effects on the survival rate of larvae, larval developmental duration, mean pupal weight and emergence rate. Corn particle had significant effect on the survival rate of larvae. Sucrose had significant effect on mean pupal weight, and yeast extract had significant effect on larval developmental duration. As the results, the optimized weight ratio of corn particle to sucrose and yeast extract was 9:16:3
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