为了解铅(Pb)在植物体内的迁移转化规律,以大花萱草、‘金娃娃’萱草、‘甜心’玉簪和东北玉簪为材料,采用土壤盆栽试验,结合细胞组分分离和化学形态提取技术,研究了这4种宿根花卉对Pb的吸收特性,分析了地上、地下部分Pb的化学结合形态及其亚细胞分布。结果表明:植株地下部分Pb含量总体高于地上部分,随Pb处理时间的延长,4种植株均出现Pb吸收峰值,但是达到最大Pb吸收值的时间有所差异; 4种植株对Pb的转运系数均减小。液泡及细胞可溶性部分是Pb在4种花卉根和叶细胞内的主要分布位点,其次是细胞壁及其残渣部分,细胞膜及细胞器部分最少。4种花卉均以HCl 提取态为主导形态,但是各形态Pb分布比例有差异,其中以东北玉簪中的乙醇提取态和水提取态所占比例较高。这说明Pb 的化学形态分布与植物种类有关,也表明东北玉簪对Pb 有较强的耐性。Four perennial flowering plants of Hemerocallis middendorfii, Hemerocallis×hybrida‘stella deoro’,Hosta‘so sweet’ and Hosta ensata were selected for a pot soil experiment combined with subcellular fractionation and chemical speciation sequential extraction techniques. The purpose was to study the accumulation characteristics, chemical forms and subcellular distribution of lead in the four plants. The results showed that the content of Pb in the underground part is more than that of in the aboveground part of the four flowers. All of the four plants emerged a maximum absorption content along with increasing the Pb treating time, but the time of reaching maximum absorption was different, and the transfer coefficient of the four flowers all reduced. A difference of Pb distribution and its chemical forms in the subcell of the four flowers was observed. Most of Pb was located in the vacuolus and cellular soluble parts, then in the cell wall and its residue and less Pb in the cell membranes and organelles. Pb in the four plants predominated HCl extractable, but the distribution forms of lead were different, the proportion of water and ethanol extractable northeast Pb was higher in Hosta ensata than that of in the other plants. This showed that the chemical form of Pb was related to the plan species, and also indicated Hosta ensata was likely to possess a higher tolerance to Pb
