OALib Journal期刊
不同美国山核桃品种花粉萌发与活力研究
DOI: 10.3969/j.issn.1000-2006.2015.04.001
Keywords: 美国山核桃 , 品种 , 离体培养条件 , 花粉萌发率 , 花粉管长度Carya illinoensis , variety , in vitro culture condition , pollen germination rate , pollen tube length
Abstract:
为了分析不同培养条件对美国山核桃花粉生活力的影响,以4个不同品种美国山核桃花粉为试验材料,研究了培养时间、培养温度、培养基pH、蔗糖、硼酸和PEG-4000浓度等对花粉离体萌发的影响。结果表明:不同培养时间对美国山核桃花粉的生活力有显著影响,适宜的培养时间为24 h; 适宜的培养温度为25～30 ℃,温度过高或者过低均会抑制花粉萌发和花粉管生长; 适宜的液体培养基pH为5.5,超过此范围均表现为抑制萌发。在一定浓度范围内,蔗糖、硼酸和PEG-4000对美国山核桃4个品种的花粉萌发和花粉管生长起促进作用,而超过阈值后起抑制作用; 培养基组分中以含20%～25%的蔗糖、0.03%的硼酸和15%的PEG-4000 为宜。In order to analyze effects of different culture conditions on pollen vitality, influences of culture time,culture temperature, pH value of culture medium,sugar,boric acid and PEG-4000 concentration on pollen germination were studied by in vitro culture of four different Carya illinoensis cultivars.The results showed that culture time had extremely significant effect on the pollen germination and the pollen length of C. illinoensis. The suitable culture time was 24 h. The culture temperature,pH value,sugar boric acid and PEG-4000 concentrations in liquid medium had obvious influence on pollen viability of the four varieties.The optimum culture temperature for all varieties was 25-30 ℃. The weak acidic environment was favorable for germination and growth of pollens, and the suitable pH value of liquid medium was 5.6.The pollen germination rate and pollen tube length reached their peaks when the sugar concentration was 20%-25%, the concentration of boric acid was over 0.03%; and the concentration of PEG-4000 was over 15%
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