亚硫酸盐蔗渣浆同步糖化发酵法产乙醇的工艺优化

亚硫酸盐蔗渣浆是一种较易水解的预处理纤维原料,经酶解可获得较高的葡萄糖得率和总糖得率。笔者以亚硫酸盐蔗渣浆为原料,利用树干毕赤酵母同步糖化发酵法生产乙醇,研究了其生产工艺,分析了温度、pH、酵母接种量、底物质量浓度和纤维素酶用量对亚硫酸盐蔗渣浆同步糖化发酵法产乙醇的影响。结果表明,利用亚硫酸盐蔗渣浆同步糖化发酵法产乙醇的优化条件为:底物质量浓度75 g/L,纤维素酶15 μmol/(min？g),酵母接种量0.13 g/L,温度36 ℃,pH 4.8。在此条件下亚硫酸盐蔗渣浆经树干毕赤酵母同步糖化发酵72 h,乙醇质量浓度达到17.89 g/L,为理论得率的55.99%。
Bagasse sulfite pulp is a kind of pretreated cellulosic material, which can be easily hydrolyzed, and high glucose and total sugar yield can be obtained after enzymatic hydrolysis. In this study, the preparation of ethanol from bagasse sulfite pulp(BSP)was investigated using Pichia stipitis by simultaneous saccharification and fermentation(SSF). Effects of temperature, pH, inoculum size, substrate mass concentration and cellulase dosage on ethanol production were investigated. The results confirmed that ethanol could be produced from BSP using P. stipitis by SSF, and the optimal conditions were as follows: 36 ℃, pH 4.8, 0.13 g/L of inoculum size, 75 g/L of substrate mass concentration, 15 μmol/(min？g)cellulose of cellulase dosage. Under the optimal conditions, 17.89 g/L of ethanol mass concentration were produced in 72 h with 55.99% of theoretical yield. The optimized process of this study could provide technical references for ethanol production from BSP by SSF