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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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‘南林895’杨pdnac1基因克隆及蛋白的亚细胞定位
DOI: 10.3969/j.issn.1000-2006.2015.03.010, PP. 50-54
Keywords: ‘南林895’杨,nac转录因子,pdnac1,亚细胞定位
Abstract:
以‘南林895’杨(populusdeltoides×p.euramericanacv‘nanlin895’)为材料,通过race-pcr技术,获得了nac1基因的全长cdna,命名为pdnac1。该序列含有1个长906bp的开放阅读框,编码302个氨基酸。该蛋白的分子质量为34.23ku,等电点为7.44。分析其氨基酸序列发现,该蛋白具有典型nac转录因子特征,nam结构域位于11～135个氨基酸之间,含有a、b、c、d、e5个亚结构域,该区域可以结合dna;而c端含有一个高度变异的转录激活区。利用杨树原生质体对pdnac1蛋白进行细胞亚定位,结果发现pdnac1不仅明显定位于细胞核中,而且在细胞膜上也有表达。但pdnac1是否具有相应的跨膜结构域尚不明确。
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