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OALib Journal期刊
ISSN: 2333-9721
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基于ssr标记的美洲黑杨杂交子代的鉴定
DOI: 10.3969/j.issn.1000-2006.2015.03.009, PP. 45-49
Keywords: 美洲黑杨,子代鉴定,ssr标记,遗传图谱构建
Abstract:
杂交子代的纯度直接影响到遗传图谱的质量,因而在遗传图谱构建前要对杂交子代进行鉴定与选择。笔者从300对ssr标记中,筛选出11对能明确显示亲本来源的标记,利用这11对标记对380株美洲黑杨杂交子代进行了鉴定。结果表明:有372个个体因同时具有亲本各1条等位基因而被认定为亲子代可用于图谱构建,其余8个个体因产生了非亲本来源或额外的等位基因,这些个体可能源于花粉污染或染色体异常,在作图过程中宜从作图群体中排除。
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