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荧光假单胞菌pseudomonasfluorescensgcm5?1a菌株的胞外木素过氧化物酶的部分纯化和鉴定

DOI: 10.3969/j.jssn.1000-2006.2010.02.036, PP. 151-152

Keywords: 荧光假单胞菌gcm5?1a,胞外木素,过氧化物酶

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Abstract:

荧光假单胞菌gcm5?1a是松材线虫携带的一种致病菌。在gcm5?1a的培养液中发现有木质素过氧化物酶的活性,同时显示很强的毒性。通过对其胞外木质素过氧化物酶的酶学性质的初步研究发现:其最适温度为35℃,最适ph为4.0。在15~35℃、ph为6.0~9.0范围内,酶活保持相对稳定。nh2oh?hcl和kcn对酶活的抑制作用分别达到88%和57%,而nan3对酶活的抑制作用只有15%左右。ca2+、mn2+和fe3+对其活性有增强作用,但是hg2+和zn2+却有中等的抑制作用。kcn和nh2oh?hcl抑制其酶活性分别达57%、88%,而nan3的抑制率仅为15%。吸收光谱显示该酶在406nm处有一个soret峰,加入1mmol/lna2s2o4后,吸光度出现了明显的下降,但soret峰没有显著移动。

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