OALib Journal期刊
ISSN: 2333-9721
费用:99美元
|
|
|
荧光假单胞菌pseudomonasfluorescensgcm5?1a菌株的胞外木素过氧化物酶的部分纯化和鉴定
DOI: 10.3969/j.jssn.1000-2006.2010.02.036, PP. 151-152
Keywords: 荧光假单胞菌gcm5?1a,胞外木素,过氧化物酶
Abstract:
荧光假单胞菌gcm5?1a是松材线虫携带的一种致病菌。在gcm5?1a的培养液中发现有木质素过氧化物酶的活性,同时显示很强的毒性。通过对其胞外木质素过氧化物酶的酶学性质的初步研究发现:其最适温度为35℃,最适ph为4.0。在15~35℃、ph为6.0~9.0范围内,酶活保持相对稳定。nh2oh?hcl和kcn对酶活的抑制作用分别达到88%和57%,而nan3对酶活的抑制作用只有15%左右。ca2+、mn2+和fe3+对其活性有增强作用,但是hg2+和zn2+却有中等的抑制作用。kcn和nh2oh?hcl抑制其酶活性分别达57%、88%,而nan3的抑制率仅为15%。吸收光谱显示该酶在406nm处有一个soret峰,加入1mmol/lna2s2o4后,吸光度出现了明显的下降,但soret峰没有显著移动。
References
[1] | guoq,guod,zhaob,etal.twocyclicdipeptidesfrompseudomonasfluorescensgcm5?1acarriedbythepinewoodnematodeandtheirtoxicitiestojapaneseblackpinesuspensioncellsandseedlingsinvitro[j].jnematol,2007,39(3):243-247.
|
[2] | guod,zhaob,lir,etal.purificationofflagellinofpseudomonasfluorescensgcm5?1acarriedbythepinewoodnematote,bursaphelenchusxylophilus,anditsinvitrotoxicitytoasuspensionofcellsofpinusthunbergii[j].russianjournalofnematology,2008,16(2):151-157.
|
[3] | kerstenpj.glyoxaloxidaseofphanerochaetechrysosporium:itscharacterizationandactivationbyligninperoxidase[j].procnatlacadsciusa,1990,81:2280-2284.
|
[4] | hammelke,jensenka,mozuchmd,etal.ligninolysisbyapurifiedligninperoxidase[j].jbiolchen,1993,268:12274-12281.
|
[5] | hammelke.extracellularfreeradicalbiochemistryofligninollyticfungi[j].newjchem,1996,20:195-198.
|
[6] | harveyp,schoemakerhe,pslmerjm.veratrylalcoholasamediatorandtheroleofradicalcationinligninbiodegradationbyphanerochaetechrysosporium[j].febsletters,1986,195:242-246.
|
[7] | tienm,kirktk.lignin?degradingenzymefromphanerochaetechrysosporium:purification,characterization,andcatalyticpropertiesofauniqueh202?requiringoxygenase[j].procnatlacadsciusa,1984,81:2280-2284.
|
[8] | hartreeef.determinationofprotein:amodificationofthelowrymethodthatgivesalinearphotometricresponse[j].analyticalbiochemistry,1972,48:422-427.第34卷第2期2010年3月南京林业大学学报(自然科学版)journalofnanjingforestryuniversity(naturalscienceedition)vol.34,no.2mar.,2010
|
[9] | morimotok,iwaslkia.roleofmonochamusalternatus(coleoptera:cerambycidae)asavectorofbursaphelenchuslignicolus(nematoda:aphelenchoididae)[j].jjpnforsoc,1972,54(6):177-183.
|
[10] | yanom.investigationonpinedeathinnagasakiprefecture[j].sanrin?koho,1913,4(suppl.):1-14.
|
[11] | zhaobg,futaik,sutherlandjr,etal.pinewiltdisease[m].newyork:springerpublishingcompany,2008.
|
[12] | myersrf.pathogenesisinpinewiltcausedbypinewoodnematode,bursaphelenchusxylophilus[j].jnematol,1988,20(2):236-244.
|
[13] | kawazuk,kanekon.asepsisofthepinewoodnematodeisolateokd?3causesittoloseitspathogenicity[j].japjnematol,1997,27(2):76-80.
|
[14] | zhaobg,wanghl,hansfetal.distributionandpathogenicityofbacteriaspeciescarriedbybursaphelenchusxylophilusinchina[j].nematology,2003,5(6):899-906.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|