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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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木聚糖酶分级对高温降解木聚糖酶水解的影响
DOI: 10.3969/j.jssn.1000-2006.2011.03.022
Keywords: 木聚糖酶,低聚木糖,木聚糖,超滤,水解
Abstract:
为提高酶解液中聚合度为2～5的低聚木糖含量，采用超滤的方法对木聚糖酶进行分级，用于高温降解木聚糖的酶水解。结果表明：木聚糖酶原酶液经超滤分级后，得到的木聚糖酶a组分（分子质量为30～50ku）、b组分（分子质量为10～30ku）和c组分（分子质量小于10ku），均能使木聚糖中聚合度较高的糖降解为聚合度为2～5的低聚木糖。木聚糖酶b组分由于富含内切木聚糖酶xyni和xynⅱ，1mg酶蛋白可以产生2？63g木二糖、1？85g木三糖、1？15g木四糖和0？46g木五糖，明显高于木聚糖酶a和c组分高温降解木聚糖的水解能力。
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