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OALib Journal期刊
ISSN: 2333-9721
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球根花卉花粉萌发过程中糖类物质及相关酶活性的变化
DOI: 10.3969/j.issn.1000-2006.2013.04.007, PP. 34-38
Keywords: 百合,唐菖蒲,花粉萌发,糖类物质,酶活性
Abstract:
为明确兰州百合和唐菖蒲花粉在离体培养中对外源糖的选择存在显著差异的原因,在定性分析这两种球根花卉成熟花粉糖类物质的基础上,对花粉萌发过程中糖类物质含量和相关酶活性进行了测定。结果发现:两种球根花卉花粉中都含有蔗糖、葡萄糖、果糖等可溶性糖,但仅兰州百合花粉中含有淀粉,且可溶性糖含量较高。以peg4000+硼酸为培养基进行花粉培养,两种花粉均有较高的萌发率;唐菖蒲花粉在仅添加peg的培养基中不能萌发,兰州百合花粉则可以萌发。在花粉萌发过程中淀粉酶活性无显著变化,但兰州百合花粉淀粉酶活性显著高于唐菖蒲花粉,蔗糖酶及乳糖酶变化显著,在花粉培养初期迅速下降。淀粉(兰州百合)、蔗糖及葡萄糖含量的变化也很显著,尤其是葡萄糖。因此,两种花粉在peg培养基中萌发力的差异,以及对外源糖的选择性都或许与花粉中糖类物质的种类、含量及酶的活性不同有关。
| [1] | speranzage,calzonicl,pacinie.occurrenceofmonosaccharidesandpolysaccaridesreservesinmaturepollengrains[j].sexplantreprod,1997,10(2):110-115.
|
| [2] | guarnierim,speranzaa,nepim,etal.ripepollencarbohydratechangeseintrachycarpusfortunei:theeffectofrelativehumidity[j].sexplantreprod,2006,19(3):117-124.
|
| [3] | stanleyrg,linskenshf.pollen[m].newyork:springer-verlag,1974.
|
| [4] | franchigg,bellanil,nepim,etal.typesofcarbohydratereservesinpollen:localization,systematicdistributionandecophysiologicalsignicance[j].flora,1996,191:143-159
|
| [5] | dickinsondavidb.rapidstarchsynthesisassociatedwithincreasedrespirationingerminatinglilypollen[j].plantphysiol,1968,43(1):1-8.
|
| [6] | zhanghq,croesaf.anewmediumforpollengerminationinvitro[j].actabotneerl,1982,31:113-119.
|
| [7] | robertsin,steadad,ockendondj,etal.pollenstigmainteractionsinbrassicaoleracea[j].theorapplgenet,1980,58(6):241-246.
|
| [8] | 张涛,黄敏.蔗糖和peg对山茶花花粉离体萌发的影响[j].北方园艺,2009(1):101-102.zhangt,huangm.effectsofsucroseandpegonpollengerminationofcamelliainvitro[j].northernhorticulture,2009(1):101-102.
|
| [9] | 张秀梅,杜丽清,谢江辉,等.高效液相色谱法测定菠萝果实中的糖分[j].食品科学,2007,28(11):450-452.zhangxm,dulq,xiejh,etal.determinationofpineapplesaccharidescomponentsbyhighperformanceliquidchromatography[j].foodscience,2007,28(11):450-452.
|
| [10] | 吴莉,周红.应用氨基柱测定奶粉中的糖分[j].武汉化工学院学报,2002,24(2):4-6.wul,zhouh.determinationofsaccharideinmilkpowderbyspherisorbnh2colume[j].journalofwuhaninstituteofchemicaltechnology,2002,24(2):4-6.
|
| [11] | bakerhg,bakeri.starchinangiospermpollengrainsanditsevolutionarysignificance[j].amjbot,1979,66:591-600.
|
| [12] | konarrn,linskenshf.physiologyandbiochemistryofthestigmaticfluidofpetuniahybrida[j].planta,1966,71(4):372-387.
|
| [13] | 裘建宇,耿兴敏,黄蓓丽.不同糖源在球根花卉花粉萌发过程中的作用[j].安徽农业大学学报,2012,39(1):129-135.qiujy,gengxm,huangbl.effectsofdifferentsugarsourcesonpollengerminationandthepollentubegrowthofbulbflowers[j].journalofanhuiagriculturaluniversity,2012,39(1):129-135.
|
| [14] | subbaiahcc.apolyethyleneglycolbasedmediumforinvitro[j].canadianjournalofbotany,1984,62(12):2473-2475.
|
| [15] | 赵宏波,陈发棣,房伟民.栽培小菊和几种菊属植物离体萌发研究[j].南京农业大学学报,2005,28(2):22-27.zhaohb,chenfd,fangwm.pollengerminationinvitroofchrysanthemumcultivarswithsmallinflorescencesandseveralspeciesofdendranthema[j].journalofnanjingagriculturaluniversity,2005,28(2):22-27.
|
| [16] | 周莉花,赵宏波.腊梅花粉活力检测方法筛选及保存时间观测[j].浙江林学院学报,2006,23(3):270-274.zhoulh,zhaohb.pollenviabilitytestofchimonanthuspraecox[j].journalofzhejiangforestrycollege,2006,23(3):270-274.
|
| [17] | 张志良,瞿伟菁,李小方.植物生理学实验指导[m].北京:高等植物出版社,2009.
|
| [18] | bakerhg,bakeri.someevolutionaryandtaxonomicimplicationsofvariationinthechemicalreservesofpollen[c]//mulcahydl,ottavianoe.pollen:biologyandimplicationsforplantbreeding.newyork:elsevierbiomedical,1983.
|
| [19] | clémentc,pacinie.antherplastidsinangiosperms[j].botrev,2001,67(1):54-73.
|
| [20] | portnoil,horovitza.sugarsinnaturalandartificialpollengerminationsubstrates[j].annbot,1977,41(1):21-27.
|
| [21] | alonib,peetm,pharrm,etal.theeffectofhightemperatureandhighatmosphericco2oncarbohydratechangesinbellpepper(capsicumannuum)polleninrelationtoitsgermination[j].physiolplant,2001,112(4):505-512
|
| [22] | nayyarh,bainst,kumars.lowtemperatureinducedfloralabortioninchickpea:relationshiptoabscisicacidandcryoprotectantsinreproductiveorgans[j].environexpbot,2005,53(1):39-47.
|
| [23] | 田翠婷,吕洪飞,王锋,等.培养基组分对青？离体花粉萌发和花粉管生长的影响[j].北京林业大学学报,2007,29(1):47-52.tianct,lühf,wangf,etal.effectsofmediumcomponentsoninvitropollengerminationandpollentubegrowthofpiceawilsoniimast[j].journalofbeijingforestryuniversity,2007,29(1):47-52.
|
| [24] | 黄蓓丽,耿兴敏,胡凤荣,等.唐菖蒲花粉离体萌发的液体培养基研究[j].安徽农业大学学报,2009,36(4):593-597.huangpl,gengxm,hufr,etal.studyontheculturesolutionforgladioluspollenvitalitytest[j].journalofanhuiagriculturaluniversity,2009,36(4):593-597.
|
| [25] | singhmb,knoxrb.invertasesofliliumpollencharacterizationandactivityduringinvitrogermination[j].plantphysiol,1984,74(3):510-515.
|
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