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OALib Journal期刊
ISSN: 2333-9721
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银杏gbggps基因的克隆及序列分析
DOI: 10.3969/j.issn.1000-2006.2013.04.002, PP. 8-12
Keywords: 银杏萜内酯,？牛儿基？牛儿基焦磷酸合成酶(ggps),蛋白质结构,生物信息学分析
Abstract:
利用race技术克隆并获得了银杏？牛儿基？牛儿基焦磷酸合成酶基因(gbggps基因)。银杏gbggps基因的cdna全长为1641bp,含有1个1176bp的可读框,编码391个氨基酸序列。生物信息学预测gbggps蛋白的分子质量为42.51ku,理论等电点为5.98,n端有叶绿体信号肽,其二级结构主要由α-螺旋和无规则卷曲组成。蛋白质同源分析表明,gbggps含有多聚异戊二烯基合成酶家族中保守的5个特征性结构域和2个富含天冬氨酸的区域。同源建模分析显示gbggps序列与薄荷ggps蛋白的三维结构及活性位点高度相似。系统进化分析表明,银杏ggps蛋白归属植物进化支,且与加拿大红豆杉、挪威云杉、北美冷杉的ggps蛋白归为同一分支。
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