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OALib Journal期刊
ISSN: 2333-9721
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白桦spl8转录因子基因的分离及转录表达分析
DOI: 10.3969/j.issn.1000-2006.2013.03.004, PP. 17-22
Keywords: 白桦,spl8基因,转录,qrt-pcr,突变体,花发育,差异表达
Abstract:
利用race和rt-pcr技术对白桦spl转录因子spl8进行克隆,获得cdna全长为1230bp,其完整的开放读码框为930bp,编码309个氨基酸,包含1个高度保守的sbp域,预测编码蛋白的分子质量为34.32ku,理论等电点为9.08。将该基因命名为bplspl8,并将它的cdna序列提交到genbank,登录号为jq354964。同源序列比对分析表明,白桦bplspl8与拟南芥atspl8、大豆gmspl8、马铃薯scspl8、葡萄vvspl8的氨基酸序列相似性分别为55.4%、73.1%、63.9%、70.8%。qrt-pcr结果显示,bplspl8基因在不同组织均有表达,但在雄花序中最高,在种子和成熟花粉中最低;bplspl8在雄花序不同发育阶段的表达量不同,另外,在雄花序发育突变体各组织中的表达水平均低于野生型。推测bplspl8在白桦雄花序发育中的特定阶段起重要作用,其异常表达可能与雄性不育有关。
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