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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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山樱花基因组大小的测定
DOI: 10.3969/j.issn.1000-2006.2014.S1.004, PP. 17-19
Keywords: 山樱花,流式细胞仪,基因组大小
Abstract:
为了测定山樱花基因组的大小,获得适合山樱花裂解的流式细胞术的最适裂解液,优化了樱属植物流式细胞术流程。结果显示,在所对比的6种裂解液中,lb01最适合樱属植物细胞裂解。以大豆(glycinemax(l.)merrill)为内标,比较待测样品和内参峰值,计算得出山樱花基因组大小为366.67mb,c值为0.375pg。可用于樱属植物的倍性鉴定。
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