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OALib Journal期刊
ISSN: 2333-9721
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？胞质和核定位a-突触核蛋白过表达对人神经母细胞瘤细胞的影响*
DOI: 10.13232/j.cnki.jnju.2013.013, PP. 109-115
Keywords: α-突角虫核蛋白,胞质定位,核定位
Abstract:
？探讨α一突触核蛋白在人神经母细胞瘤(sh-sy5y)细胞中定位过表达的影响.研究应用plvu-snca-nes(nls)-2aegfp重组慢病毒载体感染sh-sy5y细胞，通过观察绿色荧光蛋白(eg-fp)检测目的基因在细胞中的表达情况.形态观察与mtt检测相结合，分析胞质和核定位α-突角虫核蛋白过表达对sh-sy5y细胞增殖的影响，同时测定鱼藤酮、h2o2对胞质和核定位a突角虫核蛋白过表达sh-sy5y细胞活性的影响.mitotrackcr染色法测定胞质和核定位a突角虫核蛋白过表达的sh-sy5y细胞中线粒体数目的变化.结果发现，α一突触核蛋白胞质定位过表达使得sh-sy5y细胞培养96h时活性显著增强，24h,48h,96h时细胞中线粒体的数目显著增加，增长比率分别为91.18%,41.15%,50.21%;a一突触核蛋白核定位过表达的sh-sy5y细胞相对正常细胞表现出较低的细胞活性，在培养48h,72h后细胞存活率显著下降.400nmol/l鱼藤酮损伤24h后，α-突触核蛋白核定位过表达的sh-sy5y细胞活性显著高于正常细胞角藤酮损伤组.50-800?mol/lh2o2处理条件下，α一突触核蛋白的定位过表达对sh-sy5y细胞活性的影响并不显著.研究结果表明α一突触核蛋白胞质定位过表达表现出一定的神经保护作用，α一突触核蛋白核定位过表达也能使sh-sy5y细胞的活性发生改变.
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