产漆酶真菌筛选及其产酶条件的优化
Screening of laccase-producing wild macrofungi and optimization of production conditions

【目的】从11株野生大型真菌中筛选具有产漆酶活性的大型真菌，并对漆酶活性较高的菌株进行产酶发酵条件优化，为将其投入工业化生产提供参考。【方法】以采集自安徽省琅琊山的11株真菌为材料，通过平板显色反应，筛选具有产漆酶活性的大型真菌菌株，再用液体摇瓶发酵培养方法优化大型真菌产酶培养基的组成，并对优化条件进行验证。【结果】①在供试的11株野生大型真菌中，有6株具产漆酶活性，占54.5%；其中有柄树舌灵芝(Ganoderma gibbosum) CZSWXY0001具有较高的产漆酶能力，其产酶培养基的最佳碳源为蔗糖，氮源为酵母膏。②优化后的培养基配方为：蔗糖20 g/L，酵母膏 2 g/L，K2HPO4 3.2 g/L，MgSO4·7H2O 0.2 g/L，表面活性剂十二烷基硫酸钠(SDS) 3 mg/L，Cu2+浓度6 mmol/L，pH 7.0。培养基优化后,有柄树舌灵芝菌株所产漆酶活性达到496.18 U/mL，约是优化前的12.2倍。【结论】有柄树舌灵芝CZSWXY0001具有较好的产漆酶活性。
【Objective】The specific objectives of the study were to screen laccase-producing wild macrofungi from 11 strains and optimize their optimal production conditions,which could improve the industrialization of laccase-producing strains.【Method】11 wild fungi strains collected from Langya Mountain in Anhui were studied to screen strains with laccase-producing ability through the plate color reaction.Shake flask culturing method was then used to get optimal culture media and conditions.【Result】(1) 6 out of the 11 tested strains had laccase-producing activity with the ratio of 54.5%.Ganoderma gibbosum had the highest laccase-producing ability with the best carbon and nitrogen sources of sucrose and yeast extract,respectively.(2) The optimal medium was:sucrose 20 g/L,yeast 2 g/L,K2HPO4 3.2 g/L,MgSO4·7H2O 0.2 g/L,surfactant sodium dodecyl sulfate (SDS) 3 mg/L,and copper ion concentration 6 mmol/L at the pH of 7.0.Enzyme activity under the optimal conditions was 496.18 U/mL,which was 12.2 times of the original medium.【Conclusion】Ganoderma gibbosum CZSWXY0001 had good-laccase producing ability