微波辅助提取香菇多糖工艺的响应面优化
Response surface optimization of microwave-assisted extraction of polysaccharides from Lentinus edodes

【目的】优化香菇多糖的微波提取工艺，为香菇多糖的工业化生产和综合利用提供理论依据。【方法】以香菇多糖提取率为响应值，以液（mL）料(g)比(15∶1，20∶1，25∶1，30∶1，35∶1)、微波功率(500，600，700，800，900 W)及微波时间(2，4，6，8，10 min)为因素进行单因素试验。在单因素试验基础上，采用Box-Behnken响应面设计法，建立数学模型，筛选最佳提取工艺条件。【结果】通过二次回归模型响应面分析，获得香菇多糖的最佳提取工艺条件为，液料比35∶1、微波功率900 W、微波时间8.5 min；在此条件下，多糖提取率达6.49%，与最大理论预测值(6.63%)相对误差小于5%。【结论】利用Box-Behnken响应面设计法得到了香菇多糖微波提取优化工艺，该工艺方便可行。
【Objective】The microwave-assisted extraction technique for polysaccharide from Lentinus edodes was optimized to provide theoretical basis for the industrial production and comprehensive utilization of polysaccharide from Lentinus edodes.【Method】The Box-Behnken response surface method (RSM) based on mathematical model was established using polysaccharides extraction rate as response value and using the ratio of liquid （mL） to solid (g) (15∶1,20∶1,25∶1,30∶1,and 35∶1),microwave power (500,600,700,800,and 900 W) and microwave time (2,4,6,8,and 10 min) as factors to screen optimum extraction conditions for polysaccharide from Lentinus edodes.【Result】The optimal conditions obtained by RSM were:ratio of liquid to solid 35∶1,microwave power 900 W,and microwave time 8.5 min.Under these conditions,the extraction yield of polysaccharide was 6.49%,<5% less than the maximum theoretical extraction rate of 6.63%.【Conclusion】The microwave-assisted polysaccharide extraction from Lentinus edodes was optimized by Box Behnken RSM and the process was convenient and feasible