磁微粒酶联免疫吸附法测定玉米中的伏马毒素B1

Magnetic particles (MPs) were used as the support to replace enzyme-linked immunosorbent assay (ELISA) plates for the determination of fumonisin B1 (FB1) in corn. Goat anti-rabbit antibody and protein A-MP conjugates were utilized in a direct competitive ELISA format. Under optimized conditions, the limits of detection (LOD) for goat anti-rabbit antibody and protein A-MPs were 0.024 and 0.030 μg/mL, respectively. The goat anti-rabbit antibody MP conjugates exhibited a lower LOD which was only one third of that of conventional ELISA. The recoveries from spiked corn varied from 76.4% to 110.6%, with relative standard deviation (RSD) less than 11%. Overall, a sensitive and stable method for FB1 has been developed in this paper