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OALib Journal期刊
ISSN: 2333-9721
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大柴胡颗粒对四氧嘧啶致原代胰岛细胞损伤的保护作用
, PP. 1403-1408
Keywords: 大柴胡颗粒,胰岛细胞,四氧嘧啶,细胞凋亡,bcl-2,bax
Abstract:
本文研究了大柴胡颗粒(dchkl)对四氧嘧啶(axn)致胰岛细胞损伤的保护作用。采用四甲基偶氮唑盐(mtt)法检测大柴胡颗粒对胰岛细胞活性的影响;用elisa法检测其对胰岛细胞胰岛素基础分泌量和高糖刺激胰岛素分泌量的影响;采用流式细胞仪检测胰岛细胞的凋亡率,同时以rt-pcr检测胰岛细胞的抗凋亡基因(bcl-2)和促凋亡基因(bax)的表达。大柴胡颗粒能明显增强胰岛细胞的活性(p<0.01);同时高糖刺激胰岛素分泌量明显上升(p<0.01);能抑制胰岛细胞的凋亡,降低细胞的凋亡率(p<0.01);并且出现上调bcl-2基因的表达,下调bax基因表达。结果表明,大柴胡颗粒能提高细胞活性,增加胰岛素分泌,抑制胰岛细胞的凋亡并且上调抗凋亡基因表达,下调促凋亡基因的表达。大柴胡颗粒对胰岛细胞具有一定的保护作用。
| [1] | gastaldellia,ferranninie.beta-celldysfunctionandglucoseintolerance:resultsfromthesanantoniometabolism(sam)study[j].diabetologia,2003,1:31-39.
|
| [2] | huih,dottaf,dimariou,etal.roleofcaspaseintheregulationofapoptoticpancreaticisletbetacellsdeath[j].jcellphysiol,2004,200:177-200.
|
| [3] | koum,liuql.cytokine-inducedapoptosisofpancreaticβprogress[j].jpractdiabetol(实用糖尿病杂志),2006,3:55-56.
|
| [4] | sunz,hanhr,mal,etal.studyofacupunctureinhibitingisletβ-cellapoptosisintype2diabetesmellitusrats[j].chinjgerontol(中国老年学杂志),2011,3:966-967.
|
| [5] | zhangyz,chenglf,zhangdh,etal.oleanolicacidpromoteinsulinsecretionagainstglucolipotoxicityandapoptosis[j].basicclinmed(基础医学与临床),2012,11:1308-1311.
|
| [6] | chenjy,sun,chenzx.baicalinondiabeticnephropathyaceinhibition[j].gansujtcm(甘肃中医),2009,22:66-68.
|
| [7] | sunjp,jiayj,songjh,etal.studyonmolecularmechanismofinhibitoryeffectofbaicalinonproliferationofinsulinomacelllineinrats[j].chinjintegrmed(中国中西医结合杂志),2006,4:337-340.
|
| [8] | liucp,xukf,wuxh,etal.isolation,purificationandfunctionalanalysesofratislets[j].actaacadmednanjing(南京医科大学学报),2007,27:242-245.
|
| [9] | leix,zhangs,bohrera,etal.roleofcalcium-independentphospholipasea(2)βinhumanpancreaticisletβ-cellapoptosis[j].amjphysiolendocrinolmetab,2012,303:e1386-1395.
|
| [10] | cerasie.insulinproduction,insulinsecretionandtype2diabetes:β-cellatthecoreoftheproblem[j].chinjendocrinolmetabol(中华内分泌代谢杂志),2005,21:194-198.
|
| [11] | butlerae,jansonj,bonner-weirs,etal.beta-celldeficitandincreasedbeta-cellapoptosisinhumanswithtype2diabetes[j].diabetes,2003,52:102-110.
|
| [12] | mathisd,vencec,benoistc.beta-celldeathduringprogressiontodiabetes[j].nature,2001,414:792-800.
|
| [13] | donathmy,storlingj,meaedlerk,etal.inflammatorymediatorsandisletβ-cellfailure:alinkbetweentype1andtype2diabetes[j].jmolmed,2003,81:455-470.
|
| [14] | butlerae,jansonj,ritzelr,etal.acceleratedapoptosisovercomesincreasedreplicationtocauseβ-celllossindiabetesinmicetransgenicforh-iapp[j].diabetes,2002,51:a7.
|
| [15] | butlerae,jansonj,soellerwc,etal.increasedβ-cellapoptosispreventsadaptiveincreaseinβ-cellmassinmousemodeloftype2diabetesevidenceforroleofisletamyloidformationratherthandirectactionofamyloid[j].diabetes,2003,52:2304-2314.
|
| [16] | hongfz,yufw.theprotectiveeffectsoflyciumbararumpolysaccharidesonalloxan-inducedratpancreaticisletsdamage[j].jchinmedmater(中药材),2002,25:649-651.
|
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