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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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北柴胡毛状根诱导及其植株再生体系的建立
, PP. 1491-1497
Keywords: 北柴胡,发根农杆菌,毛状根,再生植株
Abstract:
本研究从设计多种诱导条件出发,探索建立稳定的柴胡毛状根诱导体系。发根农杆菌a4侵染北柴胡无菌苗叶基部,共培养3天后,转入抑菌培养,10天开始出现毛状根,经4～5周培养,转为液体摇培,获得大量毛状根,诱导产生的毛状根可再生成植株。毛状根再生成植株有两种方式:一种是液体摇培的毛状根自发脱落形成幼苗的继续培养,另一种是将毛状根产生脱落的类愈伤组织置于经筛选的再生固体培养基上再生成植株。柴胡毛状根及其植株再生体系的建立,为开展柴胡次生代谢研究,尤其是利用农杆菌介导的遗传转化研究和利用奠定了基础。由毛状根诱导出再生植株在新种质产生和品种改良选育方面具有应用价值。
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