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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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黄芩汤对溃疡性结肠炎大鼠nf-κbp65调控作用研究
, PP. 21-27
Keywords: 黄芩汤,溃疡性结肠炎,nf-κbp65,细胞因子
Abstract:
研究黄芩汤对溃疡性结肠炎(uc)大鼠细胞因子、nf-κbp65蛋白表达的影响,探讨其治疗效果及作用机制。运用复合法(三硝基苯磺酸+乙醇)制备细胞免疫反应性uc大鼠模型,大鼠随机分成正常对照组、模型组、柳氮磺胺吡啶(sasp)组和黄芩汤高、中、低剂量组(20、10、5g·kg-1)。治疗5天后评估各组大鼠饮食量、体重变化及组织损伤程度,采用griess法测定血清中no含量,elisa法测定il-6、tnf-α、pge2含量,运用免疫组化法检测nf-κbp65蛋白表达情况。结果显示,模型组大鼠饮食量及体重显著低于正常组;血清中no、il-6、tnf-α、pge2水平、结肠组织nf-κbp65蛋白表达量及组织损伤程度显著高于正常组。黄芩汤高、中剂量组大鼠上述指标均较模型组显著好转。黄芩汤对溃疡性结肠炎的治疗作用可能是通过抑制nf-κbp65通路活化,进而下调促炎细胞因子no、il-6、tnf-α和pge2表达实现的。
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