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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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超高效液相色谱串联质谱法检测三七药材中10种真菌毒素
, PP. 81-85
Keywords: 真菌毒素,超高效液相色谱串联质谱,三七
Abstract:
为保证中药三七质量和安全性,本文建立了一种同时检测三七药材中10种真菌毒素的超高效液相色谱串联质谱定量分析方法。三七药材以乙腈超声提取,多功能净化柱(hlb柱)净化,phenomenexkinetexxb-c18柱分离,用甲醇和含5mmol·l-1醋酸铵水溶液为流动相进行梯度洗脱,以电喷雾离子源正负离子模式多反应监测方式检测。结果表明,10种毒素在各自线性范围内线性关系良好,相关系数r在0.9981～1.0000之间,定量限为0.15～8.6μg·kg-1,加标回收率为73.8%～107.0%,相对标准偏差为0.10%～10.9%。该方法净化效果好、灵敏度高、重复性好,适用于基质成分复杂的三七药材中多种真菌毒素的定量检测。
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