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OALib Journal期刊
ISSN: 2333-9721
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藁本内酯对谷氨酸诱导的pc12细胞凋亡的保护作用
, PP. 162-168
Keywords: 藁本内酯,pc12细胞,谷氨酸,钙离子,细胞色素c,细胞凋亡
Abstract:
研究藁本内酯对谷氨酸诱导pc12细胞凋亡的保护作用。采用mtt比色法检测细胞存活率;annexinv-fitc/propidiumiodide(pi)双染法流式细胞术(flowcytometry,fcm)检测细胞凋亡率;fluo-3/am荧光染色法检测钙离子含量;westernblot法检测线粒体和胞浆中细胞色素c(cytochromec,cytc)及凋亡相关蛋白caspase-3、bcl-2、bax的表达。结果显示,谷氨酸具有细胞毒性,其半数抑制浓度(ic50)为15mmol·l-1;不同浓度藁本内酯(1、5、15μmol·l-1)预处理可显著提高细胞存活率。与正常对照组相比,谷氨酸单独给药组的细胞凋亡率为13.39%;藁本内酯给药组(1、5、15μmol·l-1)使细胞的凋亡率分别减少到9.06%、6.48%和3.82%。并且藁本内酯可以显著减少谷氨酸所致的钙离子内流,westernblot结果显示,藁本内酯可能通过减少线粒体cytc的释放,抑制caspase-3活性,同时升高bcl-2/bax比值来保护谷氨酸诱导的pc12细胞凋亡。结果表明,藁本内酯对谷氨酸诱导pc12细胞的凋亡具有保护作用。该保护作用可能与抑制细胞内钙离子内流及阻断cytc从线粒体释放入胞浆有关。
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