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OALib Journal期刊
ISSN: 2333-9721
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利用ssr、srap和issr分子标记构建首张丹参遗传连锁图谱
, PP. 360-366
Keywords: 丹参,遗传连锁图谱,ssr,srap,issr
Abstract:
以2个丹参(salviamiltiorrhizabge.)品系杂交得到的94株f1代植株为作图群体,利用ssr(simplesequencerepeat,简单重复序列)、srap(sequence-relatedamplifiedpolymorphism,相关序列扩增多态性标记)和issr(inter-simplesequencerepeat,内部简单重复序列)标记进行了首张丹参遗传连锁图谱的构建。该图谱包含8个连锁群,93个标记位点,覆盖长度400.1cm,平均图距4.3cm;各连锁群包括2～23个标记,遗传距离3.3～132cm。该图谱为丹参相关数量性状的基因定位、分离以及克隆奠定了重要基础。
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