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OALib Journal期刊
ISSN: 2333-9721
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基于β-香树脂醇合成酶基因snp的甘草道地性机制研究
, PP. 906-909
Keywords: 甘草,道地性,β-香树脂醇合成酶基因,单核苷酸多态性
Abstract:
本文采用pcr-sscp和测序组合技术检测了6个产地甘草的β-香树脂醇合成酶(β-as)基因的snp,并进行了snp和产地的相关性分析,探索甘草道地性形成的分子机制。结果显示,甘草β-as基因第一外显子存在1处snp,可将甘草划分为94a型、94g型和94a/g型3种基因型,其中94a型在道地产区所占比例要远远高于非道地产区,达到极显著差异(p<0.001)。本文研究证明,甘草β-as基因94位点的snp可能是导致甘草道地性形成的机制之一。
| [1] | zouyp,ges.anovelmolecularmarker-snpsanditsapplication[j].biodivsci(生物多样性),2004,11:370-382.
|
| [2] | xiejb,wangwq.thediscussionofevolutionhistorybasedonlicoricegenuineregion[j].respractchinmed(现代中药研究与实践),2009,23:35-36.
|
| [3] | wangmg,geys,chenl,etal.relationshipappraisalofglycyrrhizauralensiswithrapd[j].jwuhanbotres(武汉植物学研究),2004,22:289-293.
|
| [4] | gesj,ligm,mazy,etal.analysisongeneticdiversityofwildpopulationsoflicorice(glycyrrhizauralensisfisch.)withaflpmarkers[j].sciagricsin(中国农业科学),2009,42:47-54.
|
| [5] | kondok,shibam,yamajih,etal.speciesidentificationoflicoricewithnrdnaandcpdnageneticmarkers[j].biolpharmbull,2007,30:1497-1502.
|
| [6] | liuy,liudj,liucs,etal.mechanismofgenuinenessofliquoriceglycyrrhizauralensisbasedoncnvsofhmgr,sqs1andβ-asgene[j].actapharmsin(药学学报),2012,47:250-255.
|
| [7] | chinesepharmacopoeiacommittee.pharmacopoeiaofthepeople'srepublicofchina,part1(中华人民共和国药典2010年版1部)[m].beijing:chinamedicalsciencepress,2010:80-81.
|
| [8] | weisl,wangwq,wangjy,etal.preliminarystudyinglycyrrhizincontentanditsinfluencingfactorsofwildandcultivatedindifferentregionofchina[j].chinajchinmatermed(中国中药杂志),2012,37:1341-1345.
|
| [9] | liudj.thecorrelationshipbetweencnvsbasedonlicoricehmgr,sqs,β-asgeneandthedifferentregionsofglycyrrhizauralensis(甘草hmgr、sqs、β-as合酶基因cnvs与产地、形态的相关性研究)[d].beijing:beijinguniversityofchinesemedicine,2011.
|
| [10] | hayashih,huangp,kirakosyana.cloningandcharacterizationofacdnaencodingβ-myrinsynthaseinvolvedinglycyrrhizinandsoyaponinbiosynthesisinlicorice[j].biolpharmbull,2001,24:912-916.
|
| [11] | sunq,tonghw,wub,etal.analysisongeneticdiversityofglycyrrhizauralensisdetectedwithmorphologicalandissrmolecularmarkers[j].jplantgenres(植物遗传资源学报),2007,8:56-63.
|
| [12] | shenzy,liucs,wangxy,etal.thecorrelationanalysisbetweensnponthecodingregionofβ-asgeneandtheglycyrrhizicacidcontentofglycyrrhizauralensis[j].chinajchinmatermed(中国中药杂志),2010,35:813-816.
|
| [13] | shenzy.thecorrelationanalysisbetweenpolymorphismofβ-asgeneandsynthesisofβ-amyrin(甘草β-as基因多态性和β香树酯醇合成的相关性研究)[d].beijing:beijinguniversityofchinesemedicine,2010.
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