|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
基于高效液相色谱-质谱技术的缬沙坦作用机制的研究
, PP. 875-881
Keywords: 代谢组学,缬沙坦,hplc-ms
Abstract:
采用高效液相色谱-质谱技术(hplc-tof/ms),并结合simca-p软件进行偏最小二乘判别分析,研究缬沙坦干预后大鼠血清内源性代谢物变化,寻找其潜在生物标记物,并利用metpa平台鉴别缬沙坦在干预机体过程中相关的代谢通路,以探索缬沙坦治疗高血压病的作用机制。结果显示,给予缬沙坦连续干预4周后收缩压降低效果明显(p<0.05);缬沙坦干预后自发性高血压大鼠(shr)血清代谢模式发生改变,鉴定出4种代谢产物及其相关代谢通路,这些代谢产物与g蛋白偶联通路密切相关。本文提示代谢组学关注药物进入机体后的整体改变和微观转化,从而可以在阐明药物作用机制方面发挥积极的作用。
| [1] | oresicm.metabolomics,anoveltoolforstudiesofnutrition,metabolismandlipiddysfunction[j].nutrmetabcardiovascdis,2009,19:816-824.
|
| [2] | xuz,caixj,zengs.thoughtsandexplorationonpharmacokineticstudyofherbalmedicinesbasedonmetabonomicsandcomplexnetwork[j].actapharmsin(药学学报),2012,47:558-564.
|
| [3] | huangy,qiuxl.effectofvalsartanonplasmaaldosteroneendothelinanditsantihypertensionefficacy[j].acadjguangzhoumedcoll(广州医学院学报),2011,29:34-36.
|
| [4] | mehtapk,griendlingkk.angiotensiniicellsignaling:physiologicalandpathologicaleffectsinthecardiovascularsystem[j].amjphysiolcellphysiol,2007,292:c82-97.
|
| [5] | zhouty,renf,dengl,etal.researchprogressinphysiologicalandbiochemicalfunctionoflinolenicacid[j].guizhouagricsci(贵州农业科学),2011,39:53-58.
|
| [6] | raoml,tangm,hejy,etal.effectsofpaeoniflorinoncerebralbloodflowandthebalanceofpgi2/txa2ofratswithfocalcerebralischemia-reperfusioninjury[j].actapharmsin(药学学报),2014,49:55-60.
|
| [7] | zoubm,jiangss,lijc,etal.releaseofplasmaarachidonicacidconcentrationinhypertensionanditseffectofmodulation[j].chincritcaremed(中国危重病急救医学),1996,8:149-151.
|
| [8] | wangsz.researchonmetabonomicswithsyndrometypeofhypertensionandthemedicalintervention(高血压病中医证型及药物干预的代谢组学研究)[d].nanjing:nanjinguniversityofchinesemedicine,2010.
|
| [9] | miaoyf,kangjh,yangjc,etal.prostaglandinereceptorsinregulationofbloodpressure[j].chinpharmacolbull(中国药理学通报),2010,4:424-427.
|
| [10] | wus.metabonomicstudiesofessenceofhypothyroidismwithspleenandkidney-yangdeficiencysyndromeandtherapeuticaleffectsofsinidecoction(基于代谢组学的甲减脾肾阳虚证本质及四逆汤治疗作用研究)[d].shanghai:secondmilitarymedicaluniversity,2013.
|
| [11] | luy,aj,wangg,etal.gaschromatography/time-of-flightmassspectrometrybasedmetabonomicapproachtodifferentiatinghypertension-andage-relatedmetabolicvariationinspontaneouslyhypertensiverats[j].rapidcommunmassspectrom,2008,866:64-76.
|
| [12] | xiezh.theinfluenceofcombinedtreatmentamlodipineandvalsartanonplasmafreefattyacidsandadiponectininpatientwithessentialhypertension(氨氯地平联合缬沙坦治疗对高血压病患者血浆脂联素和游离脂肪酸的影响)[d].nanchang:secondaffiliatedhospitalofnanchanguniversity,2008.
|
| [13] | quangyy.actionandmechanismofpioglitazoneandvalsartantreatmentofnon-alcoholicfattyliverdisease(吡格列酮、缬沙坦治疗非酒精性脂肪肝病的作用机理研究)[d].changsha:xiangyahospitalofcentralsouthuniversity,2008.
|
| [14] | ajy,wanggj,haohp,etal.differentialregulationsofbloodpressureandperturbedmetabolismbytotalginsenosidesandconventionalantihypertensiveagentsinspontaneouslyhypertensiverats[j].actapharmacolsin,2010,31:930-937.
|
| [15] | nicholsonjk,connellyj,lindonjc,etal.metabonomics:aplatformforstudyingdrugtoxicityandgenefunction[j].natrevdrugdiscov,2002,l:153-161.
|
| [16] | fengzh.medicalmolecularbiologythefirstlecture:introductionofmedicalmolecularbiology[j].chinjpractinternmed(中国实用内科杂志),1994,14:694-696.
|
| [17] | luoga,wangym,liangqq,etal.traditionalchinesemedicinesystemsbiology(中医药系统生物学)[m].bengjing:sciencepress,2011:224.
|
| [18] | wangcy,zhangsp,xuy,etal.effectofsaffloryellowbonvascularendothelialcellsinjuryinducedbyangiotensin-ii[j].actapharmsin(药学学报),2012,47:811-815.
|
| [19] | hataa,namikawac,sasakim,etal.angiotensinogenasariskfactorforessentialhypertensioninjapan[j].jclininvest,1994,93:1285-1287.
|
| [20] | xiaj,wishartds.metpa:aweb-basedmetabolomicstoolforpathwayanalysisandvisualization[j].bioinformatics,2010,26:2342-2344.
|
| [21] | chongig,junch.performanceofsomevariableselectionmethodswhenmulticollinearityispresent[j].chemomintelllabsyst,2005,78:103-112.
|
| [22] | sangstert,majorh,plumbr,etal.apragmaticandreadilyimplementedqualitycontrolstrategyforhplc-msandgc-ms-basedmetabonomicanalysis[j].analyst,2006,131:1075-1078.
|
| [23] | xuhm.effectsofprostaglandine2receptorsandgproteincoupledsignaltransductiononsynoviocytesofratswithadjuvantarthritisandtheeffectoftotalglucosidesofpaeony(前列腺素e2受体及其g蛋白偶联信号转导在佐剂性关节炎大鼠滑膜细胞功能中的作用和白芍总昔对其的影响)[d].hefei:anhuimedicaluniversity,2006.
|
| [24] | fagot-campagnaa,balkaub,simond,etal.highfreefattyacidconcentration:anindependentriskfactorforhypertensionintheparisprospectivestudy[j].intjepidemiol,1998,27:808-813.
|
| [25] | shimokawah.primaryendothelialdysfunction:atherosclerosis[j].jmolcellcardiol,1999,31:23-37.
|
| [26] | denghf,lujh,luojr,etal.effectofpuerarinonimpairedendothelium-dependentrelaxationinducedbylysophosphatidylcholineinrabbitaorta[j].jxiangnanunivmedsci(湘南学院学报医学版),2007,1:17-19.
|
| [27] | colombaionil,garcia-gilm.sphingolipidmetabolitesinneuralsignalingandfunction[j].brainresbrainresrev,2004,46:328-355.
|
| [28] | yuj,novgorodovsa,chudakovad,etal.jnk3signalingpathwayactivatesceramidesynthaseleadingtomitochondrialdysfunction[j].jbiolchem,2007,282:25940-25949.
|
| [29] | caocy.nmrstudiesofangiotensinⅱtype1receptorandtheinteractionbetweenvalsartanandmimeticmembranes(缬沙坦与类膜介质的相互作用及1型血管紧张素ⅱ受体的nmr研究)[d].wuhan:huazhonguniversityofscienceandtechnology,2012.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
