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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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lc-ms/ms法同时测定人尿液中苦杏仁苷和芍药苷及其尿药排泄研究
, PP. 1330-1335
Keywords: 苦杏仁苷,芍药苷,尿药排泄,lc-ms/ms
Abstract:
建立苦杏仁苷和芍药苷尿药浓度的lc-ms/ms同时测定方法,研究健康中国受试者静脉滴注活血通络粉针剂后其体内苦杏仁苷和芍药苷的人体尿药排泄特征。尿液样品经甲醇沉淀蛋白并稀释后,采用hederaods-2色谱柱分离,流动相为乙腈-5mmol·l-1醋酸铵水溶液(含0.05%甲酸)(20:80);质谱采用turbo-ionspray电喷雾离子源和正离子多反应监测方式。尿液中杂质不干扰样品的测定,苦杏仁苷和芍药苷的标准曲线线性范围为0.03～40μg·ml-1,线性关系良好;高、中、低三种浓度的日间和日内精密度均小于15.0%;无介质效应、残留效应;稳定性良好。9名健康受试者单次静脉输注活血通络粉针6g后,苦杏仁苷和芍药苷主要以原形经尿液排出体外,平均累计排泄率分别为79.6%和48.4%。建立的lc-ms/ms分析方法适用于健康中国受试者体内苦杏仁苷和芍药苷的尿药排泄研究。
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