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OALib Journal期刊
ISSN: 2333-9721
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藤黄酸增敏trail诱导人结肠癌ht-29细胞凋亡
, PP. 1252-1257
Keywords: 肿瘤坏死因子相关凋亡诱导配体,ht-29细胞,细胞凋亡,藤黄酸
Abstract:
研究藤黄酸(ga)与肿瘤坏死因子相关凋亡诱导配体(trail)联合作用对人结肠癌ht-29细胞的影响。采用trail、ga单独及联合作用的方法诱导ht-29细胞凋亡,mtt法检测细胞增殖的抑制;琼脂糖凝胶电泳法和流式细胞术判断细胞凋亡;通过流式细胞术检测胞浆内的活性氧自由基(ros)水平;用分光光度法检测caspase-3、-8、-9相对活性;免疫印迹实验分析c-flip、chop、dr4和dr5蛋白表达水平。联合应用1μg·ml-1ga12h,显著促进40ng·ml-1trail对ht-29细胞的生长抑制和诱导凋亡作用;使细胞出现了清晰的、凋亡特有的dnaladder条带,细胞凋亡率达(45.5±4.9)%;明显升高胞浆内ros含量;显著提高chop、dr4和dr5的表达,分别至对照组的(7.38±0.41)、(5.41±0.60)和(4.85±0.31)倍,使细胞内caspase-3、-8、-9活性明显升高(p<0.05);下调抗凋亡蛋白c-flip表达,仅为对照组的(0.22±0.08)倍。ga可能是通过ros升高激活内质网应激(ers)凋亡途径、上调dr4、dr5和下调c-flip表达来增敏trail诱导人结肠癌ht-29细胞的凋亡。
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