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OALib Journal期刊
ISSN: 2333-9721
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基于cdna-srap技术筛选的atp合成酶cf1-α亚单位基因(ctl-spn)与红花刺性状紧密连锁
, PP. 1052-1059
Keywords: 红花,有刺/无刺,cdna-srap,atp合成酶
Abstract:
红花的花是传统的活血化瘀中药,但因其花序周围苞叶有刺非常不利于人工采摘。本研究采用cdna-srap技术分析了与红花苞叶刺性状紧密连锁的基因。在分别建立红花f2代cdna无刺池和有刺池的基础上,利用60对引物筛选与苞叶刺连锁的基因片段,获得6条与红花刺性状连锁的基因片段,其中基因gpy-1和gpy-2重组率低,与苞叶刺紧密连锁并成功测序。采用race技术,克隆了gpy-2cdna全长,命名为ctl-spn,生物信息学分析显示:ctl-spn全长1679bp,具有1524bp的开放阅读框,编码508个氨基酸。同源比对发现:ctl-spn表达蛋白与其他物种的atp合成酶cf1-α亚基具有很高的同源性(97%)。半定量rt-pcr分析结果显示:gpy-1和gpy-2仅在有刺f2代单株中表达。因此,红花atp合成酶cf1-α亚基基因(ctl-spn)可能直接参与了红花刺性状的形成从而提高了有刺红花的抗逆性,这一发现为红花无刺品种的选育提供了重要思路。
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