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OALib Journal期刊
ISSN: 2333-9721
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二维斑马鱼模型联合色谱技术评价朝藿定a及其代谢物宝藿苷i抗骨质疏松活性
, PP. 932-937
Keywords: 斑马鱼,骨质疏松模型,代谢模型,活性筛选,朝藿定a,宝藿苷i
Abstract:
以二维斑马鱼模型联合色谱技术筛选朝藿定a及其代谢物宝藿苷i的抗骨质疏松活性，建立高效筛选中药抗骨质疏松活性成分新方法。用斑马鱼成鱼对朝藿定a的0.5%dmso溶液进行代谢，代谢液浓缩后用lc-ms分析、hplc捕获代谢产物宝藿苷i，用斑马鱼骨质疏松模型评价朝藿定a和宝藿苷i的活性。结果表明朝藿定a和宝藿苷i均能防止泼尼松龙诱导斑马鱼骨质疏松。该方法使朝藿定a的量微代谢产物得到富集、分离分析，通过在体斑马鱼抗骨质疏松模型简单、高效的筛选朝藿定a及代谢产物宝藿苷i的抗骨质疏松活性，为早期、快速发现中药量微成分及其代谢物的抗骨质疏松活性提供新思路与方法。
| [1] | zhangdw,chengy,wangnl,etal.effectsoftotalflavonoidsandflavonolglycosidesfromepimediumkoreanumnakaiontheproliferationanddifferentiationofprimaryosteoblasts[j].phytomedicine,2008,15:55-61.
|
| [2] | wangcm,weiyj,jiaxb,etal.thezebrafishmodelofosteoporosisinducedbyprednisolone[j].worldscitechnolmodtraditchinmed(世界科学技术-中医药现代化),2013,15:509-514.
|
| [3] | weiyj,wangcm,caixt,etal.establishmentofzebrafishosteopeniamodelinducedbydexamethasone[j].actapharmsin(药学学报),2013,48:255-260.
|
| [4] | joneshs,trollopeht,hutchinsonth,etal.metabolismofibuprofeninzebrafishlarvae[j].xenobiotica,2012,42:1069-1075.
|
| [5] | hug,siuso,lis,etal.metabolismofcalycosin,anisoflavonefromastragaliradix,inzebrafishlarvae[j].xenobiotica,2012,42:294-303.
|
| [6] | lic,jiangzt,lir.identificationofchemicalconstituentsofflavonoidsintheleavesofprunuspseudocerasusl.byhplc-ms-ms[j].foodsci(食品科学),2013,34:226-230.
|
| [7] | pengs,zhangg,hey,etal.epimedium-derivedflavonoidspromoteosteoblastogenesisandsuppressadipogenesisinbonemarrowstromalcellswhileexertingananaboliceffectonosteoporoticbone[j].bone,2009,45:534-544.
|
| [8] | zhany,weiyj,wangcm,etal.evaluationofantiosteoporoticactivityformicroamounticariinandepimedinbbasedontheosteoporosismodelusingzebrafish[j].chinpharmj(中国药学杂志),2014,49:30-35.
|
| [9] | zhany,weiyj,wangcm,etal.preventiveeffectoftotalflavonoidsepimediifoliumonthezebrafishosteoporosismodelinducedbyprednisolone[j].chinhosppharmj(中国医院药学杂志),2014,34:251-255.
|
| [10] | xiejp,sunwj.progressofchemicalcompositionpharmacologicalstudyofherbaepimedii[j].straitpharmj(海峡药学),2006,18:17-20.
|
| [11] | weiyj,lip,fanh,etal.metaboliteprofilingoffourmajorflavonoidsofherbaepimdiiinzebrafish[j].molecules,2012,17:420-432.
|
| [12] | yangqx,liuyq,wangl,etal.screeningofbioactivecomponentsinepimediumforosteoporosistreatmentbymodelpopulationanalysis[j].actapharmsin(药学学报),2012,47:1205-1209.
|
| [13] | cuil,sune,wangjj,etal.preparationofepimedinsa,b,cfromepimediumbrevicornummaximusingmiddlepressurepreparativesystemcombinedautopurificationsystem[j].jnanjingunivtraditchinmed(南京中医药大学学报),2013,29:60-62.
|
| [14] | jiads,jiaxb,zhaojl,etal.preparationofbaohuosideibyenzymolysisoficariinwithcellulase[j].chintraditherbdrugs(中草药),2010:888-892.
|
| [15] | zhaowc,songlj,wenkh,etal.researchprogressoflongspurepimediumforanti-osteoporosis[j].chinamedher(中国医药导报),2012,9:21-25.
|
| [16] | maxn,gebf,chengkm,etal.mechanismsoficariininregulatingboneformationofosteoblastsandboneresorptionofosteoclasts[j].actaacadmedsin(中国医学科学院学报),2013,35:432-438.
|
| [17] | qianl,wengwj,licy,etal.researchprogressineffectoftotalflavonesofepimediuminsituonboneandcartilagecells[j].chinjbasicmedtraditchinmed(中国中医基础医学杂志),2012,18:347-349.
|
| [18] | barrettr,chappellc,quickm,etal.arapid,highcontent,invivomodelofglucocorticoid-inducedosteoporosis[j].biotechnolj,2006,1:651-655.
|
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