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OALib Journal期刊
ISSN: 2333-9721
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胡椒碱对肠易激综合征模型大鼠脑-肠5-羟色胺和突触小泡蛋白的影响
, PP. 1785-1791
Keywords: 胡椒碱,肠易激综合征,5-羟色胺,突触小泡蛋白
Abstract:
探讨胡椒碱对慢急性联合应激大鼠的抑郁样行为学和内脏敏感性的改善作用以及对脑-肠中5-羟色胺（5-ht）和突触小泡蛋白表达的影响。42只sd大鼠随机分为正常对照组、模型组、胡椒碱（12.5、25、50及100mg·kg-1）组和阳性对照组（丙咪嗪10mg·kg-1）。采用慢急性联合应激建立肠易激综合征（ibs）模型来评估动物抑郁样行为和肠道敏感性的变化，用高效液相色谱法（hplc）测定药物对大鼠海马和结肠5-ht含量的影响，用westernblotting法测定大鼠海马和结肠突触小泡蛋白的含量。与正常对照组相比，ibs模型组大鼠强迫游泳不动时间明显增加，糖水消耗量明显降低，大鼠内脏敏感性增加（p<0.05，p<0.01）；大鼠海马中5-ht的水平下降，5-羟吲哚乙酸（5-hiaa）与5-ht的比例（5-hiaa/5-ht）增加（p<0.01），而结肠中5-ht的水平升高，5-hiaa与5-ht的比例（5-hiaa/5-ht）降低（p<0.05）；大鼠海马中突触小泡蛋白的表达水平下降，结肠中表达水平反而增加（p<0.05）。胡椒碱能逆转上述引起的变化。ibs大鼠存在抑郁状态和内脏敏感性改变，脑-肠内5-ht和突触小泡蛋白表达异常，胡椒碱可改善ibs大鼠模型行为学变化，并调节5-ht和突触小泡蛋白的表达。
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