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OALib Journal期刊
ISSN: 2333-9721
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高速逆流色谱结合电喷雾多级串联质谱方法高效分离制备、鉴定五味子木脂素类成分
, PP. 78-82
Keywords: 高速逆流色谱,电喷雾多级串联质谱,五味子,木脂素
Abstract:
应用高速逆流色谱技术（hsccc）从北五味子粗提物中快速分离制备木脂素类成分。采用两种溶剂系统进行洗脱，溶剂系统分别为正己烷-乙酸乙酯-甲醇-水（9：1：5：5）及正己烷-乙酸乙酯-甲醇-水（9：1：9：5），转速900r·min-1，流速为2.0ml·min-1，从五味子粗提物一次分离制备出5种单体成分。利用电喷雾多级串联质谱技术（esi-msn）及超高效液相色谱技术（uplc）对高速逆流色谱分离产物进行结构鉴定及纯度分析。5种单体成分分别为五味子醇甲、戈米辛j、五味子醇乙、五味子酯甲和五味子甲素，纯度分别为98.74%、94.32%、99.53%、94.23%和98.68%。该方法快速、简便，分离效率高，适用于五味子木脂素类成分的制备。
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