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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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过表达hdr和ads基因对青蒿素生物合成的影响
, PP. 1346-1352
Keywords: 黄花蒿,青蒿素,羟甲基丁烯基-4-磷酸还原酶,紫穗槐二烯合成酶,转基因
Abstract:
青蒿素是从草本药用植物黄花蒿（artemisiaannual.）中分离提取的一种含有过氧桥基团结构的新型倍半萜内酯，它是世界卫生组织推荐治疗脑型疟疾和抗氯喹恶性疟疾的特效药物。本研究采用过表达青蒿素生物合成途径中关键酶基因的方法，培育出青蒿素含量提高的转基因黄花蒿。采用根癌农杆菌介导遗传转化方法，潮霉素抗性筛选获得同时转化hdr和ads两个基因的转基因黄花蒿；通过基因组pcr检测确认获得了6个共转化hdr和ads基因的转基因株系。实时荧光定量pcr检测基因表达量，结果表明转基因株系中hdr和ads基因表达量均高于非转基因植株（仅ah3株系ads基因表达量没有显著性提高）；hplc测定青蒿素含量结果表明转基因株系中青蒿素含量均高于非转基因植株。青蒿素含量最高的转基因株系ah70中青蒿素含量为非转基因对照的3.48倍。本研究实验结果表明，过表达hdr和ads基因在黄花蒿的代谢途径中具有提高青蒿素含量的作用，为进一步利用代谢工程技术培育青蒿素高产转基因黄花蒿提供了良好基础。
| [1] | zenglx,chenm,lanxz,etal.metabolicregulationoftheartemisininbiosyntheticpathwayinartemisiaannual.[j].jmedplantsres,2012,6:7-13.
|
| [2] | klaymandl.qinghaosu(artemisinin):anantimalarialdrugfromchina[j].science,1985,228:1049-1055.
|
| [3] | zhangxb,guolp,huanglq.climatesuitablerankdistributionofartemisinincontentofartemisiaannuainchina[j].actapharmsin(药学学报),2011,46:472-478.
|
| [4] | liunf,qulb,xiangbr.antitumormechanismofqinghaosuderivatives-moleculardockingstudiesofqinghaosuderivativeswithtransferring[j].actapharmsin(药学学报),2009,44:140-144.
|
| [5] | wangq,wulm,zhaoy,etal.theanticancereffectofartesunateanditsmechanism[j].actapharmsin(药学学报),2002,37:477-478.
|
| [6] | rodk,paradiseem,ouelletm,etal.productionoftheantimalarialdrugprecursorartemisinicacidinengineeredyeast[j].nature,2006,440:940-943.
|
| [7] | paddoncj,westfallpj,piteradj.high-levelsemi-syntheticproductionofthepotentantimalarialartemisinin[j].nature,2013,496:528-532.
|
| [8] | grahamia,besserk,blumers,etal.thegeneticmapofartemisiaannual.identifieslociaffectingyieldoftheantimalarialdrugartemisinin[j].science,2010,327:328-331.
|
| [9] | liub,wangh,duz,etal.metabolicengineeringofartemisininbiosynthesisinartemisiaannual.[j].plantcellrep,2010,30:689-694.
|
| [10] | pengmf,chenm,chenr,etal.thelastgeneinvolvedinthemeppathwayofartemisiaannual.:cloningandcharacterizationandfunctionalidentification[j].jmedplantsres,2011,5:223-230.
|
| [11] | kongjq,huangy,shenjh,etal.recentadvancesinthestudyofamorpha-4,11-dienesynthaseanditsmetabolicengineering[j].actapharmsin(药学学报),2009,44:1320-1327.
|
| [12] | anthonyjr,anthonylc,nowroozif,etal.optimizationofthemevalonate-basedisoprenoidbiosyntheticpathwayinescherichiacoliforproductionoftheanti-malarialdrugprecursoramorpha-4,11-diene[j].metabeng,2009,11:13-19.
|
| [13] | liaozh.molecularbiologyofthebiosyntheticpathwaysoftaxolprecursorsandmetabolicengineeringofanti-tumorterpenoidindolealkaloids(紫杉醇前体生物合成的分子生物学和抗癌萜类吲哚生物碱的代谢工程)[d].shanghai:fudanuniversity,2005.
|
| [14] | xiangle,zenglx,yuany,etal.enhancementofartemisininbiosynthesisbyoverexpressingdxr,cyp71av1andcprintheplantsofartemisiaannual.[j].plantomicsj,2012,5:503-507.
|
| [15] | zhangd,yangl,yanglx,etal.determinationofartemisinin,arteannuinbandartemisinicacidinherbaartemisiaeannuaebyhplc-uv-elsd[j].actapharmsin(药学学报),2007,42:978-981.
|
| [16] | lux,shenq,zhangl,etal.promotionofartemisininbiosynthesisintransgenicartemisiaannuabyoverexpressingads,cyp71av1andcprgenes[j].indcropprod,2013,49:380-385.
|
| [17] | pfafflmw.anewmathematicalmodelforrelativequantificationinreal-timert-pcr[j].nucleicacidsres,2001,29:45.
|
| [18] | linnf,heidmannl,saedlerh.epigeneticchangesintheexpressionofthemaizealgeneinpetuniahybrida:roleofnumbersofintegratedgenecopiesandstateofmethylation[j].molgengenet,1990,222:329-336.
|
| [19] | alamp,abdinmz.over-expressionofhmg-coareductaseandamorpha-4,11-dienesynthasegenesinartemisiaannual.anditsinfluenceonartemisinincontent[j].plantcellrep,2011,30:1919-1928.
|
| [20] | vergauwea,cammaertr,vandenberghed,etal.agrobacteriumtumefaciens-mediatedtransformationofartemisiaannual.andregenerationoftransgenicplants[j].plantcellrep,1996,15:929-933.
|
| [21] | kongjq,wangw,chengkd,etal.researchprogressesinsyntheticbiologyofartemisinin[j].actapharmsin(药学学报),2013,48:193-205.
|
| [22] | miralpeixb,rischerh,hakkinenst,etal.metabolicengineeringofplantsecondaryproducts:whichwayforward?[j].currpharmdesign,2013,19:5622-5639.
|
| [23] | nafist,akmalm,ramm,etal.enhancementofartemisinincontentbyconstitutiveexpressionofthehmg-coareductasegeneinhigh-yieldingstrainofartemisiaannual.[j].plantbiotechnolrep,2011,5:53-60.
|
| [24] | cheny,shenq,wangy,etal.thestackedover-expressionoffps,cyp71av1andcprgenesleadstotheincreaseofartemisininlevelinartemisiaannual.[j].plantbiotechnolrep,2013,7:287-295.
|
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