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OALib Journal期刊
ISSN: 2333-9721
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影响毕赤酵母高效表达重组蛋白的主要因素及其研究进展
, PP. 1644-1649
Keywords: 毕赤酵母,表达系统,重组蛋白,基因表达,分子伴侣
Abstract:
毕赤酵母是现代分子生物学研究中最重要的重组蛋白表达系统之一.该系统具有表达水平高、遗传稳定性好、可分泌表达、易于高密度发酵和成本低廉等优点.影响重组蛋白在毕赤酵母中高效表达的主要因素有基因拷贝数、密码子偏爱性、启动子、分子伴侣、信号肽、糖基化修饰和发酵工艺等.本文对影响毕赤酵母高效表达的主要因素及其研究进展进行综述,为进一步提高重组蛋白在毕赤酵母中的表达水平提供借鉴.
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