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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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人参皂苷rb3对ox-ldl诱导内皮细胞氧化应激与功能障碍的类雌激素样保护作用
, PP. 1406-1412
Keywords: 人参皂苷rb3,氧化低密度脂蛋白,氧化应激,内皮素-1,一氧化氮
Abstract:
人参皂苷rb3（ginsenosiderb3，grb3）是西洋参茎叶总皂苷（panaxquinquefoliussaponinofstemandleaf，pqs）的主要入血成分之一，既往研究显示其具有雌激素受体激动效应。本研究观察grb3对ox-ldl诱导人脐静脉内皮细胞氧化应激与功能障碍的保护作用。药物干预后检测细胞内sod、nos活性与mda含量，elisa法进行细胞培养上清液et-1与no含量的测定，realtimert-pcr方法检测细胞中enos和inos的转录水平，westernblotting方法测定丝氨酸/苏氨酸蛋白激酶akt的磷酸化水平。与ox-ldl损伤模型组相比，grb3增高细胞内sod活力，降低细胞内mda含量与总nos活性，减少细胞et-1和no合成，升高enos的转录水平及降低inos的转录水平，且能调节akt的磷酸化水平，从而发挥保护作用，给予雌激素受体抑制剂ici182780预处理后上述保护作用消失。结果提示，grb3可通过雌激素受体的介导发挥抗ox-ldl对内皮细胞的损伤作用，其作用效应与17β-雌二醇相似。
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