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OALib Journal期刊
ISSN: 2333-9721
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肿瘤靶向高分子碲化镉量子点复合纳米粒的制备及其表征
, PP. 1457-1465
Keywords: 量子点,氨基葡萄糖,聚乙二醇,精氨酸,肿瘤
Abstract:
本文采用水热法合成n-乙酰-l-半胱氨酸稳定的碲化镉量子点（cdtequantumdots，cdteqds），采用配体置换的方法制备得到氨基脱氧葡萄糖（2-amino-2-deoxy-d-glucose，dg）、聚乙二醇（polyethyleneglycol，peg）与9聚精氨酸（9-d-arginine，9r）共同修饰的碲化镉量子点（9r/dg-cdteqds）。通过紫外、荧光光谱、傅里叶红外光谱、核磁共振氢谱、高效液相-质谱联用、聚丙烯酰胺凝胶电泳和透射电子显微镜等手段对该复合量子点进行表征，并对该复合纳米粒的生物相容性、肿瘤靶向性及穿细胞膜的效果进行考察。结果表明，通过配体置换的方法可以成功构建dg、9r、peg修饰的cdteqds复合纳米粒。tem结果显示，该纳米粒分散性较好，粒径约为8～10nm。化学修饰后的cdteqds吸收峰从480nm红移至510nm，发射峰从627nm红移至659nm。通过dg、9r、peg的修饰还能进一步改善量子点的生物相容性，提高对葡萄糖转运体1高表达的肿瘤细胞的靶向性，增加量子点穿过细胞膜进入细胞浆的作用。
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