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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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微型浮游动物摄食实验？？稀释法中浮游植物负生长的可能原因分析
Keywords: 稀释法,浮游植物,负生长率,微型浮游动物摄食
Abstract:
？稀释法(dilutiontechnique)是研究微型浮游动物摄食和浮游植物生长的常用方法之一，负值浮游植物生长率是稀释实验中常见的现象。分析了造成负值生长率出现的因素，以及这些因素对实验结果的影响，并提出了防止不利影响产生的措施。负值生长率的出现不能简单地视为实验失败的标志，培养光照和温度条件、取样误差、无颗粒水污染、营养盐污染和限制等都可能造成负生长率的出现，且对实验结果的影响不同。同时，根据实验结果，演示浮游植物光适应、取样误差、无颗粒水污染和加富营养盐对稀释实验的影响。结果显示，光照条件可以改变细胞色素含量，且不同浮游植物类群对光照条件的响应不同，从而导致基于色素分析的稀释实验结果出现误差；取样混合不均，可造成取值偏低，导致浮游植物生长率估值偏低，甚至为负值，但可能不影响对摄食率的估算。另外，实验污染(无颗粒水和加富营养盐污染)往往会抑制浮游植物生长，甚至造成浮游植物死亡。因此，培养条件模拟和人为干扰控制是稀释实验成功的关键。
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