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OALib Journal期刊
ISSN: 2333-9721
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不同蛋白源对军曹鱼幼鱼碳、氮稳定同位素分馏的影响
Keywords: 军曹鱼幼鱼,酵母蛋白,玉米蛋白,碳、氮稳定同位素,同位素分馏
Abstract:
？为研究饲料中不同蛋白源对军曹鱼幼鱼碳、氮稳定同位素分馏的影响,配制3种等氮等能饲料。d1以鱼粉为蛋白源,d2和d3饲料中分别以啤酒酵母和玉米蛋白替代10%的鱼粉蛋白,投喂幼鱼24d。结果表明,啤酒酵母和玉米蛋白替代10%的鱼粉蛋白后,幼鱼的体质量增加率显著下降。随养殖时间的延长,所有处理组幼鱼的碳稳定同位素比率δ13c逐渐上升而氮稳定同位素比率δ15n逐渐下降;虽然全鱼和肌肉δ15n的变化速度存在差异,但各饲料组全鱼和肌肉的δ13c和δ15n都在24d后与饲料达到平衡。当饲料中10%的鱼粉蛋白被啤酒酵母和玉米蛋白替代之后,幼鱼肌肉和全鱼样品与饲料相比的碳同位素富集δ13c值下降,而氮同位素富集δ15n值则上升。其中全鱼δ13c从4.19‰分别下降到3.94‰和3.63‰,肌肉δ13c从4.46‰分别下降到3.98‰和3.67‰;全鱼δ15n从0.18‰分别增加到0.88‰和0.94‰,肌肉δ15n从0.18‰分别增加到0.74‰和0.87‰。军曹鱼在摄食3种不同蛋白源饲料时,其全鱼和肌肉的δ13c和δ15n的变化趋势相似,但全鱼δ15n的变化速度慢于肌肉。据此可推断,肌肉可在生态学营养级研究(长时间尺度)中代表军曹鱼的碳、氮同位素特征;但在代谢生理学研究中(短时间尺度),肌肉就无法准确反映军曹鱼全鱼的δ15n变化过程。
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