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OALib Journal期刊
ISSN: 2333-9721
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菱形藻株系md1的脂肪酸组成及其在nahco3、nacl和ph值影响下的生长特征
Keywords: 菱形藻nitzschiasp.,嗜碱特性,ph,nahco3,epa
Abstract:
？？研究了具有嗜碱特性的菱形藻nitzschiasp.(md1)株系的脂肪酸组分、含量以及不同nahco3、nacl和ph值水平下对其生长的影响。gc-ms测定结果显示,在nahco3浓度为0.2mol？l?1、nacl浓度为30g？l?1、初始ph值为9.5时,md1的epa含量占干重的3.02%,占总脂肪酸的比例为17.93%。生长测定结果表明,md1具有极强的耐碱能力,其生长适宜的nahco3浓度为0.1—0.2mol？l?1,在0.3mol？l?1的nahco3浓度下仍能良好生长;md1最适的起始ph值范围为7.5—9.5,其生长速率在ph7.5与ph9.5之间没有显著性差异(p>0.05),可以耐受ph值为11.5的高碱性环境并保持较好的生长;md1显示广盐适应性,nacl浓度在0g？l?1时获得最大比生长速率,在30—40g？l?1时对其生长没有显著影响(p>0.05),在nacl浓度30g？l?1、ph8.5—9.5及nahco30.2mol？l?1时,获得了最大比生长速率0.56d?1。由此可见,md1可望具有进行大规模培养开发epa的前景。
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