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一株微微型浮游植物的分离培养与鉴定

Keywords: 微微型浮游植物,picochlorum属,分类鉴定,18srdna

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Abstract:

?从我国南海北部大洋陆架外海域中取样分离培养的1株微微型浮游植物(scsio-8),以它为材料,对其生长特征、形态结构、生理生化特性和分类鉴定等进行了研究。研究结果表明,scsio-8为游离单细胞,细胞大小为?(2.08±0.28μm)×(1.78±0.09)μm,无鞭毛,无蛋白核,以似亲孢子方式繁殖,形成2、4个子细胞;扫描电镜观察,scsio-8具有1个细胞核、1个线粒体和1个叶绿体,形成大量淀粉粒;scsio-8适应生长的盐度范围至少为0‰―38‰,并可快速适应海淡水之间的转换;hplc分析显示scsio-8细胞含有叶绿素a、b以及顺/反式紫黄质、?-胡萝卜素和叶黄素等类胡萝卜素组分;经18srdna测序和对比分析,结合其细胞形态结构、生理生化特性,初步鉴定为picochlorumsp.scsio-8。

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