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OALib Journal期刊
ISSN: 2333-9721
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稀杯盔形珊瑚铜锌超氧化物歧化酶基因全长cdna序列的克隆与分析*
Keywords: 铜锌超氧化物歧化酶,基因克隆,序列分析,稀杯盔形珊瑚galaxeaastreata
Abstract:
？利用反转录pcr和race法获得稀杯盔形珊瑚galaxeaastreata的铜锌超氧化物歧化酶(copper-zincsuperoxidedismutase,cuznsod)基因cdna全序列,大小为730bp。5°非编译区(5°-utr)为111bp,3°-utr为188bp,开放阅读框(openreadingframe,orf)长度为471bp,编码156个氨基酸。编码的目的氨基酸序列中含有两个cuznsod蛋白家族信号,cu2+分别与his49、his51、his66和his123配位,zn2+则与his66、his74、his83和asp86配位,cys60和cys149之间形成唯一的链内二硫键。signalp3.0server预测并未发现信号肽,tmhmmserverv.2.0预测不存在跨膜结构,psortiiprediction预测位于细胞质的可能性为56.5%,推测可能是胞内cuznsod。本研究结果为进一步揭示造礁石珊瑚的抗氧化分子机制奠定了基础。
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